|本期目录/Table of Contents|

[1]黄越,陈文铎,周炜,等.复制缺陷型腺病毒PCA的构建及其对结肠癌细胞生长的影响[J].浙江理工大学学报,2013,30(06):901-905.
　HUANG Yue,CHEN Wen duo,ZHOU Wei,et al.Establishment of Replication deficient Recombinant Adenovirus PCA and Its Influence on Cell Growth of Colon Cancer[J].Journal of Zhejiang Sci-Tech University,2013,30(06):901-905.
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复制缺陷型腺病毒PCA的构建及其对结肠癌细胞生长的影响()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第30卷
期数:
2013年06期
页码:
901-905
栏目:
(自科)生物与生命科学
出版日期:
2013-11-10

文章信息/Info

Title:
Establishment of Replication deficient Recombinant Adenovirus PCA and Its Influence on Cell Growth of Colon Cancer
文章编号:
1673-3851 (2013) 06-0901-05
作者:
黄越;  陈文铎;  周炜;  董淼;  高永;  许韶威;  徐涛;  唐斌
浙江理工大学生命科学学院, 杭州 310018
Author(s):
HUANG Yue;  CHEN Wen duo;  ZHOU Wei;  GAO Yong;  DONG Miao;  XU Shao wei;  XU Tao;  TANG Bin
School of Life Sciences, Zhejiang Sci Tech University, Hangzhou 310018, China
关键词:
Ad p14 ARF;  结肠癌;  MTT;  结晶紫染色;  细胞凋亡
分类号:
Q789
文献标志码:
A
摘要:
通过同源重组方式,利用Adeasy系统成功构建携带p14ARF基因的复制缺陷型腺病毒,命名为Ad pShuttle CMV p14ARF(PCA),并研究其对结肠癌细胞株SW480及HT 29的杀伤作用。PCR方法鉴定阳性重组子病毒;MTT法检测PCA对细胞增殖的影响;Hoechst33258荧光染色法检测细胞凋亡形态学变化;结晶紫染色法定性分析PCA对两种结肠癌细胞的杀伤作用。结果显示:经目的病毒PCA作用,SW480及HT 29出现明显病变和生长抑制现象,并呈现出时间和浓度依赖性;Hoechst染色后观察到细胞内出现凋亡小体,并且数目随药物处理浓度的升高而增多。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期: 2012-12-10
基金项目: 国家自然基金资助项目(30721712)
作者简介: 黄越(1986-),女,吉林四平人,硕士研究生,主要从事天然药物抗肿瘤活性研究。
通信作者: 徐涛,电子邮箱:pkuxt@yahoo.com.cn
更新日期/Last Update: 2013-11-12