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[1]张漫莉,耿新伟,王梦婷,等.纤维素酶EGA基因在枯草芽孢杆菌中的表达及其产物性质研究[J].浙江理工大学学报,2013,30(03):389-393.
　ZHANG Manli,GENG Xinwei,WANG Mengting,et al.Research on Expression of Cellulase EGA Gene in Bacillus Subtilis and Properties of Its Product[J].Journal of Zhejiang Sci-Tech University,2013,30(03):389-393.
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纤维素酶EGA基因在枯草芽孢杆菌中的表达及其产物性质研究()

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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:: 第30卷
期数:: 2013年03期

页码:: 389-393

栏目:: (自科)生物与生命科学

出版日期:: 2013-05-10

文章信息/Info

Title:: Research on Expression of Cellulase EGA Gene in Bacillus Subtilis and Properties of Its Product

文章编号:: 1673-3851 (2013) 03-0389-05

作者:: 张漫莉; 耿新伟; 王梦婷; 蒋磊; 赵辅昆; 陈玮; 浙江理工大学蛋白质组学与分子酶学实验室, 杭州 310018

Author(s):: ZHANG Manli; GENG Xinwei; WANG Mengting; JIANG Lei; ZHAO Fukun; CHEN Wei; Laboratory of Proteomics and Molecular Enzymology, Zhejiang Sci-Tech University, Hangzhou 310018, China

关键词:: 枯草芽孢杆菌; 纤维素酶EGA; 分泌; 羧甲基纤维素钠

分类号:: Q786

文献标志码:: A

摘要:: 枯草芽孢杆菌 Bacillus subtilis是一种高效的外源蛋白表达菌株,能将目的蛋白分泌到细胞外的培养基中。从福寿螺胃液共生菌株Bacillus sp. Strain AC 1基因组中克隆纤维素酶EGA的基因序列,利用基因工程技术构建纤维素酶EGA的重组枯草芽孢杆菌表达载体pAUsp ega 。该载体含有一个强启动子和一段信号肽,经淀粉诱导能表达外源蛋白。以枯草芽孢杆菌蛋白酶缺失型菌株WB700为宿主菌,表达得到的重组纤维素酶EGA以羧甲基纤维素钠(CMCNa)为底物得到的培养基上清酶活力达到1 120 U/L。该纤维素酶在pH 6.0表现最大水解活力,最适反应温度为60℃,在酸性条件下稳定性良好,具有较好的应用前景。

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