|本期目录/Table of Contents|

[1]向超,徐向群. 响应面法优化桦褐孔菌产三萜化合物发酵培养基[J].浙江理工大学学报,2013,30(01):124-129.
　XIANG Chao,XU Xiang qun. Submerged Culture Optimization for Biomass and Triterpenoids Production by Inonotus obliquus Using Response Surface Methodology[J].Journal of Zhejiang Sci-Tech University,2013,30(01):124-129.
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 响应面法优化桦褐孔菌产三萜化合物发酵培养基()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第30卷
期数:
2013年01期
页码:
124-129
栏目:
(自科)生物与生命科学
出版日期:
2013-01-20

文章信息/Info

Title:
 Submerged Culture Optimization for Biomass and Triterpenoids Production by Inonotus obliquus Using Response Surface Methodology
文章编号:
16733851 (2013) 01012406
作者:
 向超;  徐向群
 浙江理工大学理学院, 杭州 310018
Author(s):
 XIANG Chao;  XU Xiangqun
 School of Sciences, Zhejiang SciTech University, Hangzhou 310018, China
关键词:
 ; 桦褐孔菌;  三萜化合物;  响应面法;  深层发酵
分类号:
Q815
文献标志码:
A
摘要:
    通过单因子实验筛选出桦褐孔菌发酵产三萜化合物最佳碳源和氮源分别为葡萄糖和混合氮源，采用部分因子实验得到对三萜含量有显著影响的4个组分，即葡萄糖、蛋白胨、CaCl2和酵母粉，再利用最陡爬坡实验并结合中心组合实验和响应面分析得到最佳培养基组成：葡萄糖58.9 g/L、蛋白胨2.9 g/L、CaCl2 0.5 g/L、酵母粉1.2 g/L、KH2PO41.0 g/L和MgSO4 0.2 g/L。采用该优化培养基发酵，三萜含量达到57.1 mg/g，较优化前(41.8 mg/g)和天然子实体(36.3 mg/g)分别提高了36.6%和57.3%。

参考文献/References:

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备注/Memo

备注/Memo:
 收稿日期： 2011-11-09
基金项目： 浙江省科技厅科技计划项目(2007C24016)
作者简介： 向超(1986-)，男，湖北荆门人，硕士研究生，主要从事生物技术与生物化工的研究。
通信作者： 徐向群，电子邮箱:xuxiangqun@zstu.edu.cn
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