|本期目录/Table of Contents|

[1]贾军祥,张风群,黄云仙,等. RNA结合蛋白Vigilin在翻译水平上对雌激素受体ERbeta的调控研究[J].浙江理工大学学报,2012,29(06):879-882.
　JIA Jun xiang,ZHANG Feng qun,HUANG Yun xian,et al. Translational Level Regulation of RNABindingProtein Vigilin to ERbeta[J].Journal of Zhejiang Sci-Tech University,2012,29(06):879-882.
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 RNA结合蛋白Vigilin在翻译水平上对雌激素受体ERbeta的调控研究()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第29卷
期数:
2012年06期
页码:
879-882
栏目:
(自科)生物与生命科学
出版日期:
2012-12-28

文章信息/Info

Title:
 Translational Level Regulation of RNABindingProtein Vigilin to ERbeta
文章编号:
16733851 (2012) 06087904
作者:
 贾军祥;  张风群;  黄云仙;  丁明;  赵辅昆
 浙江理工大学生命科学学院 蛋白质组学与分子酶学研究室, 杭州 310018
Author(s):
 JIA Junxiang;  ZHANG Fengqun;  HUANG Yunxian;  DING Ming;  ZHAO Fukun
 Proteomics and Molecular Biology Laboratory, School of Life Sciences,Zhejiang SciTech University, Hangzhou 310018, China
关键词:
 ; RNA结合蛋白;  雌激素受体;  翻译水平调控;  高密度脂蛋白结合蛋白;  信号通路
分类号:
Q291
文献标志码:
A
摘要:
    Vigilin是一个由14个连续的KH结构域组成的RNA结合蛋白。它可以通过与mRNA结合调控mRNA的翻译后表达水平，并能够结合脂蛋白。本实验室此前的实验中发现Vigilin参与了对雌激素信号传导通路的调控，为详细研究其调控机制，文章通过凝胶阻滞实验证明了Vigilin能在体外结合于雌激素受体3非翻译区（ERbeta 3UTR）。借助双荧光素酶报告基因实验发现，高表达Vigilin可以提高雌激素信号通路中的重要受体ERbeta的翻译效率。利用激光共聚焦显微镜和荧光蛋白定位实验，发现Vigilin在细胞内大量定位于pbody中。通过上述实验证明了Vigilin能够通过结合ERbeta 3UTR调控ERbeta的表达量，参与了细胞内雌激素信号传导通路。由于ERbeta是雌激素信号传导中非常重要的受体，因此该研究具有重要的意义。

参考文献/References:

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备注/Memo

备注/Memo:
 收稿日期： 2012-04-27
作者简介： 贾君祥（1985-），男，浙江浦江人，硕士研究生，主要从事RNA结合蛋白的功能及雌激素信号通路研究。
通讯作者： 丁明,Email:mingding@zstu.edu.cn
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