|本期目录/Table of Contents|

[1]仇晶晶,陈玮,丁明,等. 响应面法优化重组枯草芽孢杆菌发酵生产青霉素G酰化酶〖[J].浙江理工大学学报,2012,29(06):868-873.
　QIU Jing jing,CHEN Wei,DING Ming,et al. Optimization of Penicillin G Acylase Production by RecombinantBacillus Subtilis via Response Surface Analysis[J].Journal of Zhejiang Sci-Tech University,2012,29(06):868-873.
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 响应面法优化重组枯草芽孢杆菌发酵生产青霉素G酰化酶〖()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第29卷
期数:
2012年06期
页码:
868-873
栏目:
(自科)生物与生命科学
出版日期:
2012-12-28

文章信息/Info

Title:
 Optimization of Penicillin G Acylase Production by RecombinantBacillus Subtilis via Response Surface Analysis
文章编号:
16733851 (2012) 06086806
作者:
 仇晶晶;  陈玮;  丁明;  张漫莉;  赵辅昆
 浙江理工大学生命科学学院, 杭州 310018
Author(s):
 QIU Jingjing;  CHEN Wei;  DING Ming;  ZHANG Manli;  ZHAO Fukun
 School of Life Sciences, Zhejiang SciTech University, Hangzhou 310018, China
关键词:
 ; 青霉素G酰化酶;  枯草芽孢杆菌;  响应面;  发酵
分类号:
Q815
文献标志码:
A
摘要:
    利用响应面法优化重组枯草芽孢杆菌pAUBBmPGA/BS168发酵生产青霉素G酰化酶的条件。通过PlackettBurman设计法对碳源﹑氮源﹑无机盐﹑温度等19个因素对发酵产青霉素G酰化酶的影响进行评价。筛选出发酵温度和酵母膏为影响产酶的显著因素。采用了中心组合设计实验对两种显著影响因素进行了优化，应用响应面分析确定了显著因素的最佳水平。结果表明，当温度为34℃，酵母膏为8.8 g/L时，最终的酶活达到28.2 U/mL，比初始发酵条件提高了1.19倍。在最佳条件利用15 L的发酵罐对重组枯草芽孢杆菌进行扩大培养，最终酶活可达51.0 U/mL，相对于摇瓶发酵又有大幅度的提高。

参考文献/References:

 ［1］ Elander R P. Industrial production of betalactam antibiotics［J］. Applied Microbiology and Biotechnology, 2003, 61: 38592.
［2］ Anuj K Chandel, L Venkateswar Rao, M Lakshmi Narasu, et al. The realm of penicillin G acylase in βlactam antibiotics［J］. Enzyme and Microbial Technology, 2008, 42: 199207.
［3］ Skrob F, Becka S, Plhackova K, et al. Novel penicillin G acylase from Achromobacter sp. CCM4824［J］. Enzyme and Microbial Technology, 2003, 32: 738744.
［4］ 施晓疌, 王永红, 张嗣良, 等. NIPAB法测定亲霉素G酰化酶酶活力的分析和改进［J］. 中国医药工业杂志, 2008, 39(9): 659661.
［5］ 代志凯, 张翠, 阮征. 试验设计和优化及其在发酵培养基优化中的应用［J］. 微生物学报, 2010, 37(6): 894903.
［6］ 杨冀艳, 胡磊, 许杨. PlackettBurman设计和响应面法优化荷叶总黄酮的提取工艺［J］. 食品科学, 2009, 30(6): 2933.
［7］ Li Yin, Cui Fengjie, Liu Zhiqiang, et al. Improvement of xylanase production by Penicillium oxalicum ZH30 using response surface methodology［J］. Enzyme and Microbial Technology, 2007, 40: 13811388.
［8］ Douglas C Montgomery. Design and Analysis of Experiments［M］. 北京: 人民邮电出版社, 2007: 405458.
［9］ Li C, Bai J H, Cai Z L. Optimization of a cultural medium for bacteriocin production by Lactococcus using responsible surface methodology［J］. J Biotechnol, 2002, 93: 2734.
［10］ 罗璇, 钟晓凌, 王金华, 等. 红酵母产类胡萝卜素提取工艺的优化研究［J］. 生物技术, 2007, 17(4): 8688.
［11］ Flores G, Soberón X, Osuna J. Production of a fully functional, permuted singlechain penicillin G acylase［J］. Protein Science, 2004, 13: 16771683.

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备注/Memo

备注/Memo:
 收稿日期： 2012-01-10
 作者简介： 仇晶晶(1987-)，男，安徽合肥人，硕士研究生，主要从事发酵工艺的研究。
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