|本期目录/Table of Contents|

[1]何倩,杨耿兵,姚超,等. E\|cadherin启动子调控的慢病毒荧光报告载体的构建[J].浙江理工大学学报,2012,29(06):843-846.
　HE Qian,YANG Geng bing,YAO Chao,et al. Construction of Lentiviralbased System with FluorescentReporter Gene Regulated by Ecadherin Promoter[J].Journal of Zhejiang Sci-Tech University,2012,29(06):843-846.
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 E\|cadherin启动子调控的慢病毒荧光报告载体的构建()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第29卷
期数:
2012年06期
页码:
843-846
栏目:
(自科)生物与生命科学
出版日期:
2012-12-28

文章信息/Info

Title:
 Construction of Lentiviralbased System with FluorescentReporter Gene Regulated by Ecadherin Promoter
文章编号:
16733851 (2012) 06084304
作者:
 何倩;  杨耿兵;  姚超;  钱程
 浙江理工大学生命科学学院， 杭州 310018
Author(s):
 HE Qian;  YANG Gengbing;  YAO Chao;  QIAN Cheng
 School of Life Sciences, Zhejiang SciTech University, Hangzhou 310018, China
关键词:
 ; E\|cadherin;  慢病毒载体;  黄色荧光蛋白
分类号:
Q78
文献标志码:
A
摘要:
    E钙黏素(E\|cadherin)的缺失是上皮\|间质转化(EMT)的重要标志，而EMT可促进肿瘤细胞的侵润及转移。为进一步研究E\|cadherin与EMT的关系，研究构建了E\|cadherin启动子驱动的黄色荧光蛋白慢病毒表达载体，构建的载体经酶切鉴定正确；采用磷酸钙法包装出慢病毒，取病毒上清感染人肝癌PLC/PRF/5细胞株，通过荧光观察和qRT\|PCR实验结果显示E\|cadherin在靶细胞中稳定高表达。该载体的构建为深入研究E\|cadherin相关的EMT现象提供了良好的工具。

参考文献/References:

 ［1］Their J P. Epithelial\|mesenchymal transitions in tumor progression［J］. Nature Reviews Cancer, 2002, 2(6): 442\|454.
［2］ Thiery J P, Acloque H, Huang R Y J, et al. Epithelial\|mesenchymal transitions in development and disease［J］. Cell, 2009, 139(5): 871\|890.
［3］ Miyoshi H, Takahashi M, Gage F H, et al. Stable and efficient gene transfer into the retina using an HIV\|based lentiviral vector［J］. Proceedings of the National Academy of Sciences of the United States of America, 1997, 94(19): 10319\|10323.
［4］ Brabletz T, Jung A, Reu S, et al. Variable beta\|catenin expression in colorectal cancers indicates tumor progression driven by the tumor environment［J］. Proc Natl Acad Sci U S A, 2001, 98(18): 10356\|10361.
［5］ Brabletz T, Jung A, Spaderna S, et al. Migrating cancer stem cells: an integrated concept of malignant tumour progression［J］. Nature Reviews Cancer, 2005, 5(9): 744\|749.
［6］ Lee J M, Dedhar S, Kalluri R, et al. The epithelial\|mesenchymal transition: new insights in signaling, development, and disease［J］. Journal of Cell Biology, 2006, 172(7): 973\|981.
［7］ Yap A S, M S Crampton, J Hardin. Making and breaking contacts: the cellular biology of cadherin regulation［J］. Current Opinion in Cell Biology, 2007, 19(5): 508\|514.
［8］Naldini L, Blomer U, Gallay P, et al. In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector［J］. Science, 1996, 272(5259): 263\|267.

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备注/Memo

备注/Memo:
 收稿日期： 2011-12-16
基金项目： 国家自然科学基金重大项目（81090423）
作者简介： 何倩（1987- ），女，江苏镇江人，硕士研究生，主要从事肿瘤的靶向基因治疗研究。
通讯作者： 钱程，电子邮箱:cqian3184@yahoo.com.cn
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