|本期目录/Table of Contents|

[1]刘红彦,马国兴,杨力媛,等. 西兰花端粒酶分离与活性测定探究[J].浙江理工大学学报,2012,29(04):604-608.
　LIU Hong yan,MA Guo xing,YANG Li yuan,et al. Isolation and Activity Evaluation of Broccoli Telomerase[J].Journal of Zhejiang Sci-Tech University,2012,29(04):604-608.
点击复制

 西兰花端粒酶分离与活性测定探究()
分享到：

浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第29卷
期数:
2012年04期
页码:
604-608
栏目:
(自科)生物与生命科学
出版日期:
2012-08-28

文章信息/Info

Title:
 Isolation and Activity Evaluation of Broccoli Telomerase
文章编号:
16733851 (2012) 04060405
作者:
 刘红彦;  马国兴;  杨力媛;  郑洁;  刘小川
 浙江理工大学生物工程研究所, 杭州 310018
Author(s):
 LIU Hongyan;  MA Guoxing;  YANG Liyuan;  ZHENG Jie;  LIU Xiaochuan
 Bioengineering Institute, Zhejiang SciTech University, Hangzhou 310018, China
关键词:
 ; 西兰花;  端粒酶分离;  端粒酶检测;  聚乙二醇（PEG）
分类号:
Q814.1
文献标志码:
A
摘要:
     植物端粒酶以自身的RNA亚基为模板，维持着染色体末端端粒的长度，从而使植物细胞可持续分裂。本研究以西兰花花蕾为材料，采用不同分子量、不同浓度的PEG分别提取端粒酶，根据PEG吸附蛋白质的原理，从具有端粒酶活性的组织中分离并富集端粒酶。结果显示：用PEG 6000提取西兰花端粒酶，且用100 μg的酶量进行酶促反应时测得的端粒酶活性最高。以此为参数，经改进的TRAP法成功检测到：用0.2 g PEG 6000/700 μL洗脱液提取出的端粒酶活性很高。这些分离、检测技术为其它植物组织中端粒酶活性的检测和评价提供了新的途径，尤其对低活性的植物端粒酶检测具有重要意义，为深入研究端粒酶RNA的序列及结构奠定了基础。

参考文献/References:

 ［1］ Richards E J, Ausubel F M. Isolation of a higher eukaryotic telomere from Arabidopsis thaliana［J］. Cell, 1988, 53(1): 127136.
［2］ Moyzis R K, Buckingham J M, Cram L S, et al. A highly conserved repetitive DNA sequence, (TTAGGG)n, present at the telomeres of human chromosomes［J］. Proc Natl Acad Sci USA, 1988, 85(18): 66226626.
［3］ Rhyu M S. Telomeres, telomerase, and immortality［J］. J Natl Cancer Inst, 1995, 87(12): 884894.〖LL〗
［4］ Davis A J, Siu L L. Telomerase: therapeutic potential in cancer［J］. Cancer Invest, 2000, 18(3): 269277.
［5］ Blackburn E H, Greider C W, Szostak J W. Telomeres and telomerase: the path from maize, Tetrahymena and yeast to human cancer and aging［J］. Nat Med, 2006, 12(10): 11331138.
［6］ Fitzgerald M S, Riha K, Gao F, et al. Disruption of the telomerase catalytic subunit gene from Arabidopsis inactivates telomerase and leads to a slow loss of telomeric DNA［J］. Proc Natl Acad Sci USA, 1999, 96(26): 1481314818.
［7］ Denchi E L, de Lange T. Protection of telomeres through independent control of ATM and ATR by TRF2 and POT1［J］. Nature, 2007, 448(7157): 10681071.
［8］ Kim N W, Piatyszek M A, Prowse K R, et al. Specific association of human telomerase activity with immortal cells and cancer［J］. Science, 1994, 266(5193): 20112015.
［9］ Hou M, Xu D, Bjorkholm M, et al. Realtime quantitative telomeric repeat amplification protocol assay for the detection of telomerase activity［J］. Clin Chem, 2001, 47(3): 519524
［10］ 祝怀平, 凌斌. 检测端粒酶活性的PCR—ELISA方法的建立［J］. 安徽医科大学学报, 1999, 34(5): 334336.
［11］ 陈波, 梁江丽, 田晓平, 等. 水稻愈伤组织端粒酶催化特征检测［J］. 浙江理工大学学报, 2009, 26(4):597601.
［12］ Harris C A, Resau J H, Hudson E A, et al. Reduction of protein adsorption and macrophage and astrocyte adhesion on ventricular catheters by polyethylene glycol and NacetylLcysteine［J］. J Biomed Mater Res A, 2011, 98(3): 425433.
［13］ 张燕, 方力, 李天飞, 等. 聚乙二醇对烟草种子活力及幼苗保护酶活性的影响［J］. 云南农业大学学报, 2004, 19(1): 3640.
［14］ 吴稷. 蛋白质的PEG 化［J］. 生物工程进展, 1995, 15(4): 4851.

相似文献/References:

[1]陈智杰a,赵晓丽a,徐畅b,等. 有机颜料微胶囊在涂料染色中的应用及其染色效果分析[J].浙江理工大学学报,2013,30(01):1.
　CHEN Zhi jiea,ZHAO Xiao lia,XU Changb,et al. Application of Organic Pigment Microcapsule in Pigment Dyeingand Its Dyeing Results[J].Journal of Zhejiang Sci-Tech University,2013,30(04):1.
[2]詹永娟,谢维斌,姜晓云,等. 织物液态水传递性能的自动检测技术及应用[J].浙江理工大学学报,2013,30(01):6.
　ZHAN Yong juan,XIE Wei bin,JIANG Xiao yun,et al. Technology and Application of the Automatic Detection inTesting Liquid Transport Properties of Textiles[J].Journal of Zhejiang Sci-Tech University,2013,30(04):6.
[3]孙麒. 基于极大似然估计的织物图像分割[J].浙江理工大学学报,2013,30(01):12.
　SUN Qi. Textile Image Segmentation Based on theMaximum Likelihood Algorithm[J].Journal of Zhejiang Sci-Tech University,2013,30(04):12.
[4]巫静a,田彦杰b,汪澜a,等. 基于SVM理论的涤纶织物分散染料上染率模型研究[J].浙江理工大学学报,2013,30(01):21.
　WU Jinga,TIAN Yan jieb,WANG Lana,et al. Research on Modeling of DyeUptake Rate for Disperse Dyes onPolyester Fibers Based on SVM[J].Journal of Zhejiang Sci-Tech University,2013,30(04):21.
[5]周昊,徐英莲,齐素梅.微孔结构改性涤纶/棉混纺针织物服用性能的研究[J].浙江理工大学学报,2013,30(01):21.
　ZHAO Hao,XU Ying lian,QI Su mei. Research on Using Knitted Blend Fabric of Modified Polyesterby Cellular Structure and Cotton for Wearing[J].Journal of Zhejiang Sci-Tech University,2013,30(04):21.
[6]孙佳英,李艳清,章斐燕,等. 纺织结构复合材料铺层顺序设计与力学性能分析[J].浙江理工大学学报,2013,30(01):27.
　SUN Jia ying,LI Yan qing,ZHANG Fei yan,et al. Study on Layer Sequence Design and Mechanical Propertiesof Textile Structure Composites[J].Journal of Zhejiang Sci-Tech University,2013,30(04):27.
[7]丁源维,王騊,姚菊明,等. 静电纺制备TiO2/PVA复合纳米纤维及其光催化性能研究[J].浙江理工大学学报,2013,30(01):31.
　DING Yuan wei,WANG Tao,YAO Ju ming,et al. Photocatalytic Performance Investigation of TiO2/PVANanofibers Prepared by Electrospinning[J].Journal of Zhejiang Sci-Tech University,2013,30(04):31.
[8]章梦洁,伍仲,方园. 涤棉混纺织物阻燃性能的实验分析[J].浙江理工大学学报,2013,30(01):36.
　ZHANG Meng jie,WU Zhong,FANG Yang. Experimental Study on the PolyesterCotton BlendedFabrics Flame Retardancy[J].Journal of Zhejiang Sci-Tech University,2013,30(04):36.
[9]胡觉亮a,孔云鹏b,韩曙光a,等. 基于随机需求的服装供应链回购契约研究[J].浙江理工大学学报,2013,30(01):40.
　HU Jue lianga,KONG Yun pengb,HAN Shu guanga,et al. Study on Repurchase Contract in a Fashion Chainwith the Stochastic Demand[J].Journal of Zhejiang Sci-Tech University,2013,30(04):40.
[10]毛雯,阎玉秀. 针织服装供应商评价指标体系构建与权重确定[J].浙江理工大学学报,2013,30(01):46.
　MAO Wen,YAN Yu xiu. Building Evaluation Index System for Knitting Clothing Suppliersand Determining the Weight[J].Journal of Zhejiang Sci-Tech University,2013,30(04):46.

备注/Memo

备注/Memo:
 收稿日期： 2011-12-21
资金项目： 浙江省研究生创新基金（YK2010057）
作者简介： 刘红彦(1986-)，女，山西霍州市人，硕士研究生，研究方向为端粒与端粒酶。
通讯作者： 刘小川，电子信箱：xcliu@zstu.edu.cn
更新日期/Last Update: