|本期目录/Table of Contents|

[1]苏虹,胡贲,徐科,等. TGF\|β1对肝癌细胞系Hep3B中干性相关基因表达的影响[J].浙江理工大学学报,2012,29(01):125-128.
　SU Hong,HU Ben,XU Ke,et al. The Effect of TGF\|β1 on Gene Expression Related toStemness on Liver Cell Line Hep3B[J].Journal of Zhejiang Sci-Tech University,2012,29(01):125-128.
点击复制

 TGF\|β1对肝癌细胞系Hep3B中干性相关基因表达的影响()
分享到：

浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第29卷
期数:
2012年01期
页码:
125-128
栏目:
(自科)生物与生命科学
出版日期:
2012-02-28

文章信息/Info

Title:
 The Effect of TGF\|β1 on Gene Expression Related toStemness on Liver Cell Line Hep3B
文章编号:
16733851 (2012) 01012504
作者:
 苏虹;  胡贲;  徐科;  何倩;  姚超;  钱程;  刘立
 浙江理工大学生命科学学院 新元医药研究所， 杭州 310018
Author(s):
 SU Hong;  HU Ben;  XU Ke;  HE Qian;  YAO Chao;  QIAN Chen;  LIU Li
 Xin Yuan institute of Medicine and Biotechnology, School of Life Sciences,Zhejiang Sci\|Tech University, Hangzhou 310018, China
关键词:
 ; TGF\|β1;  Hep3B;  EMT;  干性基因
分类号:
R73\|362
文献标志码:
A
摘要:
    有研究表明TGF\|β1可以诱导诸多上皮来源的癌细胞和正常细胞发生EMT并使其功能发生改变。实验就TGF\|β1对肝癌细胞系Hep3B的作用展开研究：通过CCK8检测TGF\|β1 对Hep3B细胞增殖的影响，RT\|PCR实验检测TGF\|β1 处理后细胞中EMT及干性相关基因的表达变化。结果表明：TGF\|β1 对Hep3B细胞的增殖无抑制作用；TGF\|β1处理Hep3B细胞6d后EMT相关基因的mRNA表达水平并无显著改变，但TGF\|β1 可上调Hep3B细胞干性基因〖WTBX〗Oct\|4,Klf\|4,Nanog,C\|myc〖WTBZ〗的表达，并下调分化基因〖WTBX〗albumin〖WTBZ〗的表达。结果提示TGF\|β1一定程度上影响肝癌细胞系的干性基因表达，但并不一定是以发生EMT为前提的。

参考文献/References:

 ［1］ Roberts A B, Wakefield L M. The two faces of transforming growth factor\|β in carcinogenesis［J］. PNAS, 2003, 100(6): 8621\|8631.
［2］ Gotzmann J. Hepatocytes convert to a fibroblastoid phenotype through the cooperation of TGF\|β1 and Ha\|Ras steps towards invasiveness［J］. Mutation Research/Reviews in Mutation Research, 2004, 566(1): 9\|20.
［3］ Sendural A, Guo Wenjun. The epithelial\|mesenchymal transition generates cells with properties of stem cells［J］. Cell, 2008, 133(5): 704\|715.
［4］ Hiroaki, Ikushima, Tomoki Todo, et al. Autocrine TGF\|β signaling maintains tumorigenicity of glioma\|Initiating cells through sry\|related HMG\|Box factors［J］. Cell Stem Cell, 2009, 5(7): 504\|514.
［5］ You Hanning, Ding Wei, Bart R C. Epigenetic regulation of cancer stem cell marker CD133 by transforming growth factor\|β［J］. Hepatalogy, 2010, 49(5): 1635\|1645.
［6］ Thiery, Paul Jean. Epithelial\|mesenchymal transitions in tumour progression［J］. Curr Opin Cell Biol, 2003, 15(6): 740\|746.
［7］ Wu X Z, Chen D. Origin of hepatocellular carcinoma: role of stem cells［J］. Gastroenterol Hepatol, 2006, 21(7): 1093\|1098.
［8］ Li C, Heidt D G, Dalerba P, et al. Identification of pancroatie cancer stem cells［J］. Cancer Res, 2007, 67(3): 1030\|1037.
［9］ Bolos V, Peinado H. The transcription factor slug represses E\|cadherin expression and induces epithelial to mesenchymal transitions: a comparison wit h Snail and E47 repressors［J］. Cell Sci, 2003, 34(7): 499\|511.

相似文献/References:

[1]陈智杰a,赵晓丽a,徐畅b,等. 有机颜料微胶囊在涂料染色中的应用及其染色效果分析[J].浙江理工大学学报,2013,30(01):1.
　CHEN Zhi jiea,ZHAO Xiao lia,XU Changb,et al. Application of Organic Pigment Microcapsule in Pigment Dyeingand Its Dyeing Results[J].Journal of Zhejiang Sci-Tech University,2013,30(01):1.
[2]詹永娟,谢维斌,姜晓云,等. 织物液态水传递性能的自动检测技术及应用[J].浙江理工大学学报,2013,30(01):6.
　ZHAN Yong juan,XIE Wei bin,JIANG Xiao yun,et al. Technology and Application of the Automatic Detection inTesting Liquid Transport Properties of Textiles[J].Journal of Zhejiang Sci-Tech University,2013,30(01):6.
[3]孙麒. 基于极大似然估计的织物图像分割[J].浙江理工大学学报,2013,30(01):12.
　SUN Qi. Textile Image Segmentation Based on theMaximum Likelihood Algorithm[J].Journal of Zhejiang Sci-Tech University,2013,30(01):12.
[4]巫静a,田彦杰b,汪澜a,等. 基于SVM理论的涤纶织物分散染料上染率模型研究[J].浙江理工大学学报,2013,30(01):21.
　WU Jinga,TIAN Yan jieb,WANG Lana,et al. Research on Modeling of DyeUptake Rate for Disperse Dyes onPolyester Fibers Based on SVM[J].Journal of Zhejiang Sci-Tech University,2013,30(01):21.
[5]周昊,徐英莲,齐素梅.微孔结构改性涤纶/棉混纺针织物服用性能的研究[J].浙江理工大学学报,2013,30(01):21.
　ZHAO Hao,XU Ying lian,QI Su mei. Research on Using Knitted Blend Fabric of Modified Polyesterby Cellular Structure and Cotton for Wearing[J].Journal of Zhejiang Sci-Tech University,2013,30(01):21.
[6]孙佳英,李艳清,章斐燕,等. 纺织结构复合材料铺层顺序设计与力学性能分析[J].浙江理工大学学报,2013,30(01):27.
　SUN Jia ying,LI Yan qing,ZHANG Fei yan,et al. Study on Layer Sequence Design and Mechanical Propertiesof Textile Structure Composites[J].Journal of Zhejiang Sci-Tech University,2013,30(01):27.
[7]丁源维,王騊,姚菊明,等. 静电纺制备TiO2/PVA复合纳米纤维及其光催化性能研究[J].浙江理工大学学报,2013,30(01):31.
　DING Yuan wei,WANG Tao,YAO Ju ming,et al. Photocatalytic Performance Investigation of TiO2/PVANanofibers Prepared by Electrospinning[J].Journal of Zhejiang Sci-Tech University,2013,30(01):31.
[8]章梦洁,伍仲,方园. 涤棉混纺织物阻燃性能的实验分析[J].浙江理工大学学报,2013,30(01):36.
　ZHANG Meng jie,WU Zhong,FANG Yang. Experimental Study on the PolyesterCotton BlendedFabrics Flame Retardancy[J].Journal of Zhejiang Sci-Tech University,2013,30(01):36.
[9]胡觉亮a,孔云鹏b,韩曙光a,等. 基于随机需求的服装供应链回购契约研究[J].浙江理工大学学报,2013,30(01):40.
　HU Jue lianga,KONG Yun pengb,HAN Shu guanga,et al. Study on Repurchase Contract in a Fashion Chainwith the Stochastic Demand[J].Journal of Zhejiang Sci-Tech University,2013,30(01):40.
[10]毛雯,阎玉秀. 针织服装供应商评价指标体系构建与权重确定[J].浙江理工大学学报,2013,30(01):46.
　MAO Wen,YAN Yu xiu. Building Evaluation Index System for Knitting Clothing Suppliersand Determining the Weight[J].Journal of Zhejiang Sci-Tech University,2013,30(01):46.

备注/Memo

备注/Memo:
 收稿日期： 2011-01-17
基金项目： 国家973基金资助项目（2010CB529406）
作者简介： 苏虹(1986-)，女，山西省晋城人，硕士研究生，主要从事肿瘤基因治疗方面的研究。
通讯作者： 刘立,电子邮箱:liuli7001@ yahoo.com.cn
更新日期/Last Update: