|本期目录/Table of Contents|

[1]胡贲,苏虹,徐科,等.携带EGFP报告系统的五型腺病毒对肝癌干细胞的感染效率[J].浙江理工大学学报,2012,29(01):107-110.
　HU Ben,SU Hong,XU Ke,et al. Study on the Efficiency of 5\|type Adenovirus Which Carrying aEGFP Report System Infection with Liver Stem Cells[J].Journal of Zhejiang Sci-Tech University,2012,29(01):107-110.
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携带EGFP报告系统的五型腺病毒对肝癌干细胞的感染效率()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第29卷
期数:
2012年01期
页码:
107-110
栏目:
(自科)生物与生命科学
出版日期:
2012-02-28

文章信息/Info

Title:
 Study on the Efficiency of 5\|type Adenovirus Which Carrying aEGFP Report System Infection with Liver Stem Cells
文章编号:
16733851 (2012) 01010704
作者:
 胡贲;  苏虹;  徐科;  姚超;  何倩;  钱程;  刘立
 浙江理工大学生命科学院, 杭州 310018
Author(s):
 HU Ben;  SU Hong;  XU Ke;  YAO Chao;  HE Qian;  QIAN Cheng;  LIU Li
 School of Life Sciences, Zhejiang Sci\|Tech University, Hangzhou 310018, China
关键词:
 ; 五型腺病毒;  肝癌干细胞;  EMT;  CAR受体
分类号:
Q786
文献标志码:
A
摘要:
    利用携带EGFP报告系统的五型腺病毒（Ad5\|EGFP）作为工具，以发生上皮\|间质转化（epithelial\|mesenchymal transition,EMT）前后的肝癌细胞系Huh7为研究对象，采用流式细胞仪、荧光定量PCR等技术开展腺病毒对发生EMT前后的肝癌细胞系Huh7的感染效率的对比研究。实验结果表明：Ad5\|EGFP对上皮生产因子β1（TGF\|β1）处理前的Huh7细胞系感染率比TGF\|β1处理后的细胞系感染率更高。同时Ad5\|EGFP的主要受体\|CAR在肝癌细胞系Huh7发生EMT后表达有所降低。证实了肿瘤干细胞更难被腺病毒侵染，为了适应临床治疗的需要，腺病毒作为基因治疗的载体需要进一步被改造。

参考文献/References:

［1］ Sendurai A, Guo Wenjun, Weinberg, et al. The epithelial\|mesenchymal transition generates cells with properties of stem cells［J］. Cell, 2008, 133: 704\|715.
［2］ Beier D, Hau P, Proescholdt M, et al. CD133(+)and CD133(\|) glioblastoma\|derived cancer stem cells show differential growth characteristics and molecular profiles［J］. Cancer Res, 2007, 67(9): 4010\|4015．
［3］ Espada J, Calvo M B, Dfaz\|Prado S, et al. Wnt signaling and cancer stem cells［J］. Clin Transl Oneol, 2009, 11(7): 411\|427．
［4］ Liu S, Dontu G, Mantle I D, et al. Hedgehog signaling and Bmi\|1 regulate self\|renewal of normal and malignant human mammary stem ceils［J］. Cancer Res, 2006, 66(12): 6063\|6071．
［5］ Molofsky A V, Pardal R, Washita T, et al. Bmi\|1 dependence distinguishes neural stem cell self\|renewal from progenitor proliferation［J］. Nature, 2003, 425(6961): 962\|967．
［6］ Ponti D, Costa A, Zaffaroni N, et al. Isolation and in vitro propagation of tumorigenic breast cancer cells with stem／progenitor cell propertiesl ［J］. Cancer Res, 2005, 65(13): 5506\|5512．
［7］ Borovski T, Vermeulen L, Spriek M R, et al. One rene\|gade cancer stem cell［J］. Cell Cycle, 2009, 8(6): 803\|812．
［8］ Robey R W, To K K, Polgar O, et al. ABCG2: perspective［J］. Adv Drug Deliv Rev, 2009, 61(1): 3\|13．
［9］ Burger H, van Tol H, Boersma A W, et al. Imatinib mesylate(STI57 1)is a substrate for the breast cancer resistance protein(BcRP)／ABcG2 drug pump［J］. Blood, 2004, 104 (9): 2940\|2950．

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备注/Memo

备注/Memo:
收稿日期： 2011-01-18
基金项目： 国家973课题资助项目（2010CB529406）
作者简介： 胡贲(1986-)，男，安徽省枞阳人，硕士研究生，主要从事肿瘤基因治疗方面的研究。
通讯作者： 刘立,电子邮箱:liuli7001@ yahoo.com.cn
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