|本期目录/Table of Contents|

[1]黄青红,姚超,胡徐庞,等. 棉酚在肝癌细胞中作用机制的研究[J].浙江理工大学学报,2012,29(01):103-106.
　HUANG Qing\|hong,YAO Chao,HU Xu\|pang,et al. Study on the Mechanism of Activity on Hepatocarcinoma by Gossypol[J].Journal of Zhejiang Sci-Tech University,2012,29(01):103-106.
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 棉酚在肝癌细胞中作用机制的研究()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第29卷
期数:
2012年01期
页码:
103-106
栏目:
(自科)生物与生命科学
出版日期:
2012-02-28

文章信息/Info

Title:
 Study on the Mechanism of Activity on Hepatocarcinoma by Gossypol
文章编号:
16733851 (2012) 01010304
作者:
 黄青红;  姚超;  胡徐庞;  王刚;  杨耿兵;  刘立;  钱程;  魏旭斌
 浙江理工大学生命科学学院， 杭州 310018
Author(s):
 HUANG Qing\|hong;  YAO Chao;  HU Xu\|pang;  WANG Gang;  YANG Geng\|bing;  LIU Li;  QIAN Cheng;  WEI Xu\|bin
 School of Life Sciences, Zhejiang Sci\|Tech University, Hangzhou 310018, China
关键词:
 ; 棉酚;  肝癌;  凋亡;  Bcl\|2
分类号:
R73\|362
文献标志码:
A
摘要:
    棉酚是棉花中倍半萜(十五碳)的法尼基焦磷酸环化后合成的次生代谢产物。近来有研究显示棉酚具有抗肿瘤效果。本研究通过MTT、Hoechst染色、Western blot等生化技术开展棉酚对肝癌细胞系Bel\|7404的增殖抑制及促凋亡作用研究。MTT实验结果表明5mmol棉酚即对肝癌细胞增殖具有强烈抑制作用，Hoechst染色表明5mmol/L棉酚即可对肝癌细胞具有促凋亡作用，Western blot结果表明棉酚能下调Bcl\|2基因的表达。棉酚对肝癌细胞系Bel\|7404具有很好的抑制增殖和促凋亡的效果。

参考文献/References:

 ［1］ Keller P A, Birch C, Leach S P, et al. Novel pharmacophore\|based methods reveal gossypol as a reversetranscriptase inhibitor［J］. Mol Graph, 2003, 36(21): 365\|366.
［2］ Assikis V, Simons J W. Novel therapeutic strategies for androgen\|independent prostate cancer［J］. Semin Oncol, 2004（31）: 26\|32.
［3］ Meng Y, Li Y. Gossypol and its combination with imatin induce apoptosis in human chronic myeloid leukemic cells［J］. Leuk Lymphoma, 2007, 48(11) : 2204\|2212.
［4］ 王瑾, 王向红, 周淑雅, 等. 棉籽酚诱导结肠癌细胞系凋亡的作用［J］. 中国癌症杂志, 2000, 10 (4): 319\|322.
［5］ Shidaifat F, Canatan H, KulP S K, et al. Telomerse inhibitors in anticancer therapy: gossypol as a potential telomerase inhibitor［J］. Anticancer Res, 1997, 17(2A): 1003\|1009.
［6］ Reed J C. Bcl\|2 family proteins: strategies for overcoming chemo\|resistance in cancer［J］. Adv Pharmacol, 1997, 41: 31\|32.
［7］ Reed J C, Jurgensmeier J M, Matsuyama S. Bcl\|2 family proteins and mitochondria［J］. Biochim Biophys Acta, 1998, 1366: 27\|37.
［8］ Korsmeyer S J. Bcl\|2 gene family and the regulation of programmed cell death［J］. Cancer Res, 1999, 59: 1693\|1700.
［9］ Meng Yang, Tang Wen\|hua, Dai Liu\|yao, et al. Natural BH3 mimetic (\|)\|gossypol chemosensitizes humanprostate cancer via Bcl\|xL inhibition accompanied byincrease of Puma and Noxa ［J］. Mol Cancer Ther, 2008, 7(7): 192\|202.

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备注/Memo

备注/Memo:
 收稿日期： 2011-01-17
基金项目： 国家自然科学基金项目（30872984）
作者简介： 黄青红（1986—），男，浙江萧山人，硕士研究生，主要从事肿瘤的靶向基因治疗研究。
通讯作者： 魏旭斌，电子邮箱：runnerupstone@sohu.com
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