|本期目录/Table of Contents|

[1]徐科,胡贲,苏虹,等. 免疫细胞联合腺病毒Ad205\|IL\|15对肝癌细胞的杀伤研究[J].浙江理工大学学报,2012,29(01):99-102.
　XU Ke,HU Ben,SU Hong,et al. Anti\|Tumor Efficacy by Immune Cells Combined with AdenovirusAd205\|IL\|15 in HepatOcelIuIar Carcinoma Cells[J].Journal of Zhejiang Sci-Tech University,2012,29(01):99-102.
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 免疫细胞联合腺病毒Ad205\|IL\|15对肝癌细胞的杀伤研究()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第29卷
期数:
2012年01期
页码:
99-102
栏目:
(自科)生物与生命科学
出版日期:
2012-02-28

文章信息/Info

Title:
 Anti\|Tumor Efficacy by Immune Cells Combined with AdenovirusAd205\|IL\|15 in HepatOcelIuIar Carcinoma Cells
文章编号:
16733851 (2012) 01009904
作者:
 徐科;  胡贲;  苏虹;  胡徐庞;  刘佳;  杨智;  刘立;  钱程;  魏旭斌
 浙江理工大学新元医药研究所， 杭州 310018
Author(s):
 XU Ke;  HU Ben;  SU Hong;  HU Xu\|pang;  LIU Jia;  YANG Zhi;  LIU Li;  QIAN Cheng;  WEI Xu \|bin
 Xinyuan Institute of Medicine and Biotechnology, Zhejiang Sci\|Tech University,Hangzhou 310018, China
关键词:
 ; 腺病毒Ad205\|IL\|15;  CIK;  NK92;  过继免疫;  荧光素酶报告基因
分类号:
Q786
文献标志码:
A
摘要:
    在已有腺病毒Ad205\|IL\|15的基础上尝试与过继免疫细胞CIK、NK92等联合开展对肝癌细胞的杀伤性研究。首次通过荧光化学发光的原理构建出携带表达萤光素酶报告基因的肝癌细胞系Huh7\|Luc、MHCC97H\|Luc来检测细胞的存活率。结果表明：免疫细胞联合Ad205\|IL\|15对Huh7\|Luc、MHCC97H\|Luc两株肝癌细胞表现出了较高协同杀伤作用。证明将免疫细胞与腺病毒联合可以相互弥补各自治疗的不足之处，提高了抗肿瘤效果。同时，由于操作方便，荧光素酶报告基因测定法也为检测细胞存活率提供了新的技术手段。

参考文献/References:

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备注/Memo

备注/Memo:
 收稿日期： 2011-01-17
基金项目： 国家自然科学基金国际合作重大项目（81020108026）
作者简介： 徐科（1985-），男，浙江兰溪人，硕士研究生，主要从事肿瘤基因病毒治疗方面的研究。
通讯作者： 魏旭斌，电子邮箱：runnerupstone@sohu.com
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