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[1]徐辉,徐珏,陈成集,等. 人源血管紧张素转化酶\|N结构域基因片段在毕赤酵母中的表达[J].浙江理工大学学报,2011,28(04):611-615.
 XU Hui,XU Jue,CHEN Cheng ji,et al. Expression of Human Angiotensin Converting EnzymeN Domain in Pichia Pastoris[J].Journal of Zhejiang Sci-Tech University,2011,28(04):611-615.
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 人源血管紧张素转化酶\|N结构域基因片段在毕赤酵母中的表达()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第28卷
期数:
2011年04期
页码:
611-615
栏目:
(自科)生物与生命科学
出版日期:
2011-08-31

文章信息/Info

Title:
 Expression of Human Angiotensin Converting EnzymeN Domain in Pichia Pastoris
文章编号:
16733851 (2011) 04061105
作者:
 徐辉 徐珏 陈成集 丁明 许传莲
 血管紧张素转化酶\|N结构域; RT\|PCR; 毕赤酵母
Author(s):
 XU Hui XU Jue CHEN Chengji DING Ming XU Chuanlian
 School of Life Sciences, Zhejiang SciTech Universtry, Hangzhou 310018, China
分类号:
Q78
文献标志码:
A
摘要:
    利用RT\|PCR方法从食管癌细胞TE\|1中反转出cDNA,PCR扩增得到血管紧张素转化酶N结构域(ACE\|N)基因片段,构建pPIC9K\|ACE\|N表达载体,转化毕赤酵母GS115,阳性克隆再次电转,并从转化菌株中筛选出多拷贝转化子进行表达。经Ni2+亲和层析柱对表达的蛋白进行纯化,获得的目的蛋白表达量为0.11 g/L,其纯度大于99%。为今后选择性抑制血管紧张素转化酶两个同源结构域的体外研究奠定基础。

参考文献/References:

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备注/Memo

备注/Memo:
 收稿日期: 2010-11-02
作者简介: 徐辉(1986-),男,浙江衢州人,硕士研究生,主要从事生物化学与分子生物学研究。
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