|本期目录/Table of Contents|

[1]路巧然,吴欣欣,贾晓渊,等. 卵巢癌病人腹水细胞的培养及其分化研究[J].浙江理工大学学报,2011,28(05):809-813.
 LU Qiao ran,WU Xin xin,JIA Xia yuan,et al. Research on the Culture and Differentiation of Ovarian Cancer Ascites Cells[J].Journal of Zhejiang Sci-Tech University,2011,28(05):809-813.
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 卵巢癌病人腹水细胞的培养及其分化研究()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第28卷
期数:
2011年05期
页码:
809-813
栏目:
(自科)生物与生命科学
出版日期:
2011-10-30

文章信息/Info

Title:
 Research on the Culture and Differentiation of Ovarian Cancer Ascites Cells
文章编号:
16733851 (2011) 05080905
作者:
 路巧然 吴欣欣 贾晓渊 陈磊 陈侃
 浙江理工大学生命科学学院新元医学与生物技术研究所, 杭州 310018
Author(s):
 LU Qiaoran WU Xinxin JIA Xiayuan CHEN Lei CHEN Kan
 Xinyuan Institute of Medicine and Biotechnology, School of Life Sciences, Zhejiang SciTech University, Hangzhou 310018, China
关键词:
 肿瘤干细胞 卵巢癌病人腹水细胞 表面标志
分类号:
R737.31
文献标志码:
A
摘要:
    肿瘤干细胞是指能分化出不同表型、自我更新和不断分化的肿瘤细胞群,具有与干细胞相似的特征。文章通过流式细胞术分析从人卵巢癌腹水分离所得的细胞表面蛋白的分化特征,发现这些腹水细胞具有部分干细胞表面标志(CD44+,CD90+,β2M+)蛋白,但却不表达CD133和CD34,且体外培养2周后,腹水细胞表面标志发生了变化,这些结果为卵巢癌干细胞研究提出了新的启示。

参考文献/References:

 [1] Reya T, Morrison S J, Clarke M F, et al. Stem cells, cancer and cancer stem cells[J]. Nature, 2001, 414(6859): 105111.
[2] Cannistra S A. Cancer of the ovary[J]. N Engl J Med, 2004, 3(51): 25192529.
[3] Pecorelli S, Favalli G, Zigliani L, et al. Cancer in women[J]. Int J Gynaecol Obstet, 2003, 8(2): 369379.
[4] Godwin A K, Testa J R, Hamilton T C. The biology of ovarian cancer development[J]. Cancer, 1993, 7(1): 530536.
[5] Ness R B, Cottreau C. Possible role of ovarian epithelial inflammation in ovarian cancer[J]. Natl Cancer Inst, 1999, 9(1): 14591467.
[6] Lee J, Kotliarova S, Kotliarov Y, et al. Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumor than do serumcultured cell lines[J]. Cancer cell, 2006, 9(5): 391403.
[7] Liu G, Yuan X, Zeng Z, et al. Analysis of gene expression and chemoresistance of CD133+ cancer stem cells in glioblastoma[J]. Mol Cancer, 2006, 12(5): 6367.
[8] Wang J C, Dick J E. Cancer stem cells: lessons from leukemia[J]. Trends Cell Biol, 2005, 1(5): 494501.
[9] Hope K, Jin L, Dick J E. Acute myeloid leukemia originates from a hierarchy of leukemic stem cell classes that differ in selfrenewal capacity[J]. Nat Immunol, 2004, 3(5): 738743.
[10] Bonnet D, Dick J E. Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell[J]. Nat Med, 1997, 3(7): 730737.
[11] Singh S K, Clarke I D, Terasaki M, et al. Identification of a cancer stem cell in human brain tumors[J]. Cancer Res, 2003, 63(18): 58215828.
[12] Jemal A, Murray T, Ward E, et al. Cancer statistics[J]. CA Cancer J Clin, 2005, 55(1): 1030.
[13] Zhang Shu, Balch C, Michael W, et al. Identification and characterization of ovarian Cancerinitiating cells from primary human tumors[J]. Cancer Res, 2008, 68(11): 43114320.
[14] Counter C, Gupta J, Harley C, et al. Telomerase activity in normal leukocytes and in hematologic malignancies[J]. Blood, 1995, 8(5): 23152320.
[15] Kim N W, Piatyszek M A, Prowse K R, et al. Specific association of human telomerase activity with immortal cells and cancer[J]. Science, 1994, 26(6): 20112015.
[16] Shay J. Telomerase in human development and cancer[J]. Cell Physiol, 1997, 17(3): 266270.
[17] Hosen N, Park C Y, Tatsumi N, et al. CD34 is aleukemic stem cellspecific marker in human acute myeloid leukemia[J]. Pro Natl Acad Sci USA, 2007, 104(26): 1100811013.
[18] Beier D, Hau P, Proescholdt M, et al. CD133+ and CD133- glioblastoma derived cancer stem cels show diferential growth characteristics and molecular profiles[J]. Cancer Res, 2007, 67(9): 40104015.
[19] AlHajj M, Wicha M S, BenitoHemandez A, et al. Prospecfive identification of tumorigenic breast cancer cells[J]. Proc Natl Acad Sci USA, 2003, 100(7): 39833988.
[20] Houghton J M, Stoicov C, Nomura S. Gastric cancer originating from bone marrowderivad cells[J]. Science, 2004, 306(5701): 15681571.
[21] Li C, Heidt D G, Simeone D M, et al. Identification of pancreatic cancer stem cells[J]. Cancer Res, 2007, 67(5): 10301037.
[22] 裴雪涛. 干细胞实验指南[M]. 北京: 北京科学出版社, 2006.

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备注/Memo

备注/Memo:
 收稿日期: 2010-10-29
基金项目: 国家自然科学基金项目(30801379);浙江省教育厅科研项目(20060593);浙江省重中之重学科开放基金项目(SWYX0812);浙江理工大学科研启动基金项目(0916819Y)
作者简介: 路巧然(1984-),女,河北石家庄人,硕士研究生,主要从事肿瘤的靶向基因病毒治疗研究。
通讯作者: 陈侃,电子邮箱:chenkan_xjtu@163.com
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