|本期目录/Table of Contents|

[1]胡徐庞a,李伟a,曹跃芬b,等. 真核表达HsamiR1基因及其检测方法的建立[J].浙江理工大学学报,2011,28(05):783-788.
　HU Xu panga,LI Weia,CAO Yue fenb,et al. Eukaryotic Expression of HsamiR1 Gene and Establishment of Its Detection Method[J].Journal of Zhejiang Sci-Tech University,2011,28(05):783-788.
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 真核表达HsamiR1基因及其检测方法的建立()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第28卷
期数:
2011年05期
页码:
783-788
栏目:
(自科)生物与生命科学
出版日期:
2011-10-30

文章信息/Info

Title:
 Eukaryotic Expression of HsamiR1 Gene and Establishment of Its Detection Method
文章编号:
16733851 (2011) 05\|078306
作者:
胡徐庞a;  李伟a;  曹跃芬b;  张璇a;  黄青红a; 徐科a;  杨耿兵a;  魏旭斌a;  钱程a;  刘立a
 浙江理工大学生命科学学院， a. 新元医药研究所； b. 生物工程研究所， 杭州 310018
Author(s):
 HU Xupanga;  LI Weia;  CAO Yuefenb;  ZHANG Xuana;  HUANG Qinghonga;  XU Kea;  YANG Gengbina;  WEI Xubina;  QIAN Chenga;  LIU Lia
 Zhejiang SciTech University, a. Xinyuan Institute of Medicine and Biotechnology; b. Institute of Bioengineering, Hangzhou 310018, China
关键词:
 ; HsamiR1载体;  Taqman探针检测;  pcDNAHsamiR1;  pEGFPC1CM1
分类号:
Q786
文献标志码:
A
摘要:
    获得HsamiR1的方法主要由人工合成，需耗费很高的成本与时间。文章通过将HsamiR1前体部分连接至pcDNA3.0，可实现HsamiR1基因的高表达。构建含有HsamiR1基因表达前体的表达载体pcDNAHsamiR1和含有与HsamiR1基因完全互补序列的检测载体pEGFPC1CM1。通过共转染pcDNAHsamiR1和pEGFPC1CM1检测HsamiR1基因的表达，并采用Taqman探针法定量检测HsamiR1基因表达水平，成功构建了可高效表达HsamiR1基因的真核生物质粒pcDNAHsamiR1，并首次建立起HsamiR1基因的检测系统pEGFPC1CM1，该检测系统的建立将为今后HsamiR1在肿瘤细胞中的研究提供准确、快捷的检测方法。

参考文献/References:

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［3］ Ambs S, Prueitt R L, Yi M, et al. Genomic profiling of microRNA and messenger RNA reveals deregulated microRNA expression in prostate cancer［J］. Cancer Res, 2008, 68(15): 61626170.
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［5］ Zeng Y, Cai X, Cullen B R. Use of RNA polymeraseⅡ to transcribe artificial microRNAs［J］. Methods Enzymol, 2005, 392: 371380.
［6］ Mansfield J H, Harfe B D, Nissen R, et al. MicroRNAresponsive sensor ransgenes uncover Hoxlike and other developmentally regulated patterns of vertebrate microRNA expression［J］. Nat Genet, 2004, 36(10): 10791083.
［7］ Felli N, Fontana L, Pelosi E, et al. MicroRNA221 and 222 inhibt normal erythropoiesis and erythroleukemic cell growth via kit receptor downmodulation［J］. Proc Natl Acad Sci, 2005, 102(50): 1808121808.
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备注/Memo

备注/Memo:
 收稿日期： 2010-12-20
基金项目： 国家重点基础发展计划资助项目（973）(2010CB529406)
作者简介： 胡徐庞（1983-），男，浙江金华人，硕士研究生，从事肿瘤基因治疗研究。
通讯作者： 刘立，电子邮箱：liuli7001@yahoo.com.cn
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