|本期目录/Table of Contents|

[1]陈磊,范丽,李新,等. 蛋白酶体抑制剂MG132诱导KG\|1细胞凋亡的机制研究[J].浙江理工大学学报,2011,28(04):597-600.
　CHEN Lei,FAN Li,LI Xin,et al. Study on the Mechanism of Apoptosis by Proteasome Inhibitor MG132 in Acute Myeloid Leukeima Cell Line KG1[J].Journal of Zhejiang Sci-Tech University,2011,28(04):597-600.
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 蛋白酶体抑制剂MG132诱导KG\|1细胞凋亡的机制研究()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第28卷
期数:
2011年04期
页码:
597-600
栏目:
(自科)生物与生命科学
出版日期:
2011-08-31

文章信息/Info

Title:
 Study on the Mechanism of Apoptosis by Proteasome Inhibitor MG132 in Acute Myeloid Leukeima Cell Line KG1
文章编号:
16733851 (2011) 04059705
作者:
 陈磊;  范丽;  李新;  谢国良;  何国清;  路巧然;  吴欣欣;  李恭楚
 浙江理工大学生命科学学院 新元医学与生物技术研究所, 杭州 310018
Author(s):
 CHEN Lei;  FAN Li;  LI Xin;  XIE Guoliang;  HE Guoqing;  LU Qiaoran;  WU Xinxin;  LI Gongchu
 Xinyuan Institute of Medicine and Biotechnology, School of Life Sciences, Zhejiang SciTech University, Hangzhou 310018, China
关键词:
 ; 蛋白酶体抑制剂;  KG\|1细胞;  细胞凋亡;  NF\|κB;  PARP
分类号:
Q789
文献标志码:
A
摘要:
    研究蛋白酶体抑制剂MG132对人急性髓性白血病细胞株KG\|1的致凋亡作用及其对细胞内信号传导因子NF\|κB与凋亡蛋白PARP表达的影响。采用CCK\|8法检测MG132对KG\|1细胞增殖的抑制作用；利用Hoechst33342染色法，从形态学上观察凋亡的KG\|1细胞；Western blot检测NF\|κB活性及与凋亡相关的蛋白变化情况。CCK\|8法检测结果表明，MG132能明显抑制KG\|1细胞生长，并呈现时间依赖性和浓度依赖性；Hoechst染色和Western blot检测实验证明，MG132诱导KG\|1细胞发生凋亡的同时，NF\|κB活性明显降低，细胞凋亡蛋白PARP降解为P89蛋白。以上结果显示，MG132能诱导KG\|1细胞凋亡，其机制可能与MG132抑制NF\|κB信号转导通路，下调NF\|κB表达继而增加凋亡蛋白PARP剪切有关。

参考文献/References:

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备注/Memo

备注/Memo:
 收稿日期： 2010-06-25
基金项目： 国家自然科学基金(30801379)；浙江省“生物医学工程”重中之重学科开放基金(SWYX0804，SWYX0812)
作者简介： 陈磊(1984-)，男，浙江舟山人，硕士研究生，主要从事肿瘤的靶向基因病毒治疗研究。
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