|本期目录/Table of Contents|

[1]娄军,欧阳川,郑明珠,等. SIK1在巨噬细胞中调控IL\|6及IL\|12表达的研究[J].浙江理工大学学报,2011,28(04):591-596.
　LOU Jun,OU Yang chuan,ZHENG Ming zhu,et al. Regulation of IL6 and IL12 Expression by SIK1 Protein in Macrophages[J].Journal of Zhejiang Sci-Tech University,2011,28(04):591-596.
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 SIK1在巨噬细胞中调控IL\|6及IL\|12表达的研究()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第28卷
期数:
2011年04期
页码:
591-596
栏目:
(自科)生物与生命科学
出版日期:
2011-08-31

文章信息/Info

Title:
 Regulation of IL6 and IL12 Expression by SIK1 Protein in Macrophages
文章编号:
16733851 (2011) 04059106
作者:
 娄军1;  欧阳川2;  郑明珠2;  姚韵靓2;  王珞2;  仇庆1;  周秀梅1
 1. 浙江理工大学生命科学学院新元医学与生物技术研究所, 杭州 310018; 2. 浙江大学医学院免疫研究所, 杭州 310058
Author(s):
 LOU Jun1;  OU Yangchuan2;  ZHENG Mingzhu2;  YAO Yunliang2;  WANG Luo2;  QIU Qing1;  ZHOU Xiumei1
 1. Xinyuan Institute of Medicine and Biotechnology, School of Life Sciences, Zhejiang SciTech University, Hangzhou 310018, China; 2. Institute of Immunology, School of Medicine, Zhejiang University, Hangzhou 310058, China
关键词:
 ; SIK1;  LPS刺激;  IL\|6;  IL\|12
分类号:
R392
文献标志码:
A
摘要:
     通过LPS（lipopolysaccharide）刺激巨噬细胞，发现盐诱导激酶（Salt Inducible Kinase 1,SIK1）基因的表达随着刺激时间呈现一定的变化趋势。提示SIK1在免疫系统中可能具有潜在的功能。实验构建了Sik1基因过表达的腺病毒载体Ad\|Sik1，通过感染RAW264.7细胞过表达Sik1基因，采用荧光定量PCR、ELISA及Western Blot等方法，分别从mRNA及蛋白质水平探讨SIK1与免疫炎症因子IL\|6(Interleukin\|6)及IL\|12(Interleukin\|12)之间的关系。实验结果表明：SIK1能够上调免疫炎症因子IL\|6、IL\|12的表达。这将为免疫调节的研究及一些免疫相关疾病的临床诊断与治疗提供一定的理论依据。

参考文献/References:

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备注/Memo

备注/Memo:
 收稿日期： 2010-10-09
基金项目： 国家自然科学基金(30800093)
作者简介： 娄军(1984-)，男，湖北荆州人，硕士研究生，主要从事肿瘤的靶向基因治疗研究。
通讯作者： 周秀梅，电子邮箱：zhouxiumei824@163.com
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