|本期目录/Table of Contents|

[1]金鲁宁,李林芳,钱其军. 携带Herceptin与MICA胞外结构域融合基因的重组腺病毒的构建及鉴定[J].浙江理工大学学报,2011,28(01):106-110.
　JIN Lu ning,LI Lin fang,QIAN Qi jun. Construction and Identification of a Recombinant Adenovirus Vector Carrying a Fusion Gene Composed of Heceptin and the ECD of MICA[J].Journal of Zhejiang Sci-Tech University,2011,28(01):106-110.
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 携带Herceptin与MICA胞外结构域融合基因的重组腺病毒的构建及鉴定()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第28卷
期数:
2011年01期
页码:
106-110
栏目:
(自科)生物与生命科学
出版日期:
2011-02-28

文章信息/Info

Title:
 Construction and Identification of a Recombinant Adenovirus Vector Carrying a Fusion Gene Composed of Heceptin and the ECD of MICA
文章编号:
16733851 (2011) 01010605
作者:
 金鲁宁1;  李林芳2;  钱其军1; 2
 1. 浙江理工大学生命科学学院， 杭州 310018； 2. 第二军医大学东方肝胆外科医院病毒基因治疗实验室， 上海 200438
Author(s):
 JIN Luning1;  LI Linfang2;  QIAN Qijun1; 2
 1. School of Life Sciences, Zhejiang SciTech University, Hangzhou 310018, China; 
2. Laboratory of Viral and Gene Therapy, Eastern Hepatobiliary Surgical Hospital, Second Military Medical University, Shanghai 200438, China
关键词:
 ; Herceptin;  MICA;  腺病毒
分类号:
Q786
文献标志码:
A
摘要:
     通过构建能表达抗体Herceptin与MICA胞外结构域的融合蛋白的重组腺病毒，为进一步联合NK细胞的治疗研究奠定基础。采用OverlapPCR的方法获得Herceptin与MICA胞外结构域的融合基因，并将融合基因连接到载体pDC339上。接着再利用酶切及PCR方法鉴定载体及重组病毒基因的正确性，并通过ELISA和Western Blot检测融合蛋白的表达情况，最后经间接免疫荧光分析（IFA）检测融合蛋白的结合特性。构建的载体酶切后经电泳鉴定证实构建成功，重组病毒DNA的PCR显示病毒携带了Herceptin的轻链基因、重链基因及MICA胞外结构域的基因；ELISA和Western Blot证实重组腺病毒成功地表达出了目的融合蛋白；IFA实验表明融合蛋白能与SKOV3细胞表面的Her2受体特异性地结合。最后成功地构建了能表达具有正常结合特性的抗体Herceptin与MICA胞外结构域的融合蛋白的重组腺病毒，为之后与NK细胞的联合治疗研究奠定基础。

参考文献/References:

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备注/Memo

备注/Memo:
 收稿日期： 2010-01-08
基金项目： 国家新药创制重大专项课题（2009ZX09103687）
作者简介： 金鲁宁（1986-），男，浙江温州人，硕士研究生，主要从事肿瘤基因治疗方面的研究。
通讯作者： 钱其军，电子邮箱:qianqj@163.com
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