|本期目录/Table of Contents|

[1]李恩书,彭新荣,钱其军. 无血清条件下LIF对小鼠胚胎干细胞增殖及多能性的影响[J].浙江理工大学学报,2011,28(01):96-100.
　LI En shu,PENG Xin rong,QIAN Qi jun. Effect of LIF on the Mouse Embryo Stem Cells under SerumFree Condition[J].Journal of Zhejiang Sci-Tech University,2011,28(01):96-100.
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 无血清条件下LIF对小鼠胚胎干细胞增殖及多能性的影响()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第28卷
期数:
2011年01期
页码:
96-100
栏目:
(自科)生物与生命科学
出版日期:
2011-02-28

文章信息/Info

Title:
 Effect of LIF on the Mouse Embryo Stem Cells under SerumFree Condition
文章编号:
1673\|3851 (2011) 01\|0096\|05
作者:
 李恩书1;  彭新荣2;  钱其军1; 2
 1． 浙江理工大学新元医学与生物技术研究所， 杭州 310018； 2． 第二军医大学东方肝胆外科医院病毒治疗实验室， 上海 200438
Author(s):
 LI Enshu1;  PENG Xinrong2;  QIAN Qijun1; 2
 1. Xinyuan Institute of Medicine and Biotechnology, Zhejiang SciTech University, Hangzhou 310018, China; 2. Eastern Hepatobiliary Hospital VirusGene Therapy Laboratories, The Second Military Medical University, Shanghai 200438, China
关键词:
 ; 李恩书1;  彭新荣2;  钱其军1; 2
分类号:
Q813.11
文献标志码:
A
摘要:
    在无血清条件下培养KES小鼠胚胎干细胞，研究LIF在无血清条件下对小鼠胚胎干细胞增殖及多能性维持的影响。联合使用小分子物质SU5402、PD184352和CHIR99021或单独使用LIF培养小鼠胚胎干细胞，检测无血清条件下LIF对小鼠胚胎干细胞自我更新能力和全能性的影响。结合使用极限稀释法培养KES细胞单克隆，统计不同培养体系下单克隆的形成率。结果显示：无血清条件下，单独使用LIF，不能维持小鼠胚胎干细胞的自我更新能力和全能性；使用含有3种小分子药物 SU5402， PD184352和CHIR99021（3i）的无血清培养液可以维持小鼠胚胎干细胞的自我更新，传代60代以上仍可保持全能性；添加LIF的3i培养系统显著提高了小鼠胚胎干细胞的单克隆形成率。表明在无血清条件下，LIF不能单独维持小鼠胚胎干细胞的多能性。3i培养体系可以维持胚胎干细胞的全能性而不需要LIF；3i条件下，LIF可以显著促进胚胎干细胞体外增殖和单克隆形成。

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备注/Memo

备注/Memo:
 收稿日期： 2010-01-08
基金项目： 国家自然科学基金海外杰出青年项目（30728028）
作者简介： 李恩书（1985-），女，浙江杭州人，硕士研究生，主要从事胚胎干细胞及iPS研究。
通讯作者： 钱其军，电子邮箱：qianqj@163.com
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