|本期目录/Table of Contents|

[1]陈翠,全丽丽,徐向群.TritonX-100对液体深层发酵桦褐孔菌产活性多糖的影响[J].浙江理工大学学报,2017,37-38(自科6):888-892.
 CHEN Cui,QUAN Lili,XU Xiangqun.Effect of TritonX-100 on the Production of Active Polysaccharides  of  Inonotus Obliquus under Submerged Liquid Fermentation[J].Journal of Zhejiang Sci-Tech University,2017,37-38(自科6):888-892.
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TritonX-100对液体深层发酵桦褐孔菌产活性多糖的影响()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第37-38卷
期数:
2017年自科6期
页码:
888-892
栏目:
出版日期:
2017-11-10

文章信息/Info

Title:
Effect of TritonX-100 on the Production of Active Polysaccharides  of  Inonotus Obliquus under Submerged Liquid Fermentation
文章编号:
1673-3851 (2017) 06-0888-05
作者:
陈翠全丽丽徐向群
浙江理工大学理学院,杭州 310018
Author(s):
CHEN Cui QUAN Lili XU Xiangqun
School of Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China
关键词:
桦褐孔菌多糖TritonX-100抗氧化活性
分类号:
Q538
文献标志码:
A
摘要:
探讨表面活性剂TritonX-100对桦褐孔菌液体深层发酵产活性多糖的影响,利用醇沉法得到胞外多糖和胞内多糖,通过测定菌丝体量和胞内外多糖产量,明确TritonX-100对多糖的促进作用。进一步探讨TritonX-100的最佳添加时间及浓度,得出在第0 d添加0.1% TritonX-100,促进效果最显著。多糖的DPPH自由基清除率测定结果表明,TritonX-100也促进桦褐孔菌多糖的抗氧化活性。通过对多糖化学成分以及单糖组成的分析,证明多糖中的糖、蛋白含量及单糖的组成与DPPH自由基清除率具有一定关联性。以上结果为进一步深入研究桦褐孔菌多糖奠定理论基础,从而建立一种桦褐孔菌发酵多糖的高产、高活性的液体深层发酵制备技术。

参考文献/References:

[1] SONG Y, HUI J, KOU W, et al. Identification of Inonotus obliquus and analysis of antioxidation and antitumor activities of polysaccharides[J]. Current Microbiology,2008,57(5):454-462.
[2] DU X, MU H M, ZHOU S, et al. Chemical analysis and antioxidant activity of polysaccharides extracted from Inonotus obliquus sclerotia[J]. International Journal of Biological Macromolecules,2013,62(12),691-696.
[3] ZHENG W, MIAO K, LIU Y, et al. Chemical diversity of biologically active metabolites in the sclerotia of Inonotus obliquus and submerged culture strategies for upregulating their production[J]. Applied Microbiology and Biotechnology,2010,87(4):1237-1254.[4] XU X, QUAN L, SHEN M. Effect of chemicals on production, composition and antioxidant activity of polysaccharides of Inonotus obliquus[J]. International Journal of Biological Macromolecules,2015,77(3):143-150.
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[6] MALINOWSKA E, KRZYCKOWSKI W, LAPIENIS G, et al. Improved simultaneous production of mycelial biomass and polysaccharides by submerged culture of Hericiumerinaceum optimization using a central composite rotatable design (CCRD)[J]. Journal of Industrial of Microbioloy and Biotechnology,2009,36(12):1513-1527.
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备注/Memo

备注/Memo:
收稿日期: 2016-04-18
网络出版日期: 2017-08-07
基金项目: 浙江省自然科学基金项目(LY16B020013)
作者简介: 陈翠(1993-),女,安徽六安人,硕士研究生,主要从事生物技术和生物分析化学方面的研究
通信作者: 徐向群,E-mail:xuxiangqun@zstu.edu.cn
更新日期/Last Update: 2017-11-17