|本期目录/Table of Contents|

[1]钟云平,姚晨雪,宋国龙,等.纳米羟基磷灰石携载lefty 1基因对人乳腺癌细胞的杀伤效应[J].浙江理工大学学报,2016,35-36(自科3):450-457.
　ZHONG Yunping,YAO Chenxue,SONG Guolong,et al.Killing Effect of Nanohydroxyapatite Loading Lefty 1 Gene on Human Breast Carcinoma Cells[J].Journal of Zhejiang Sci-Tech University,2016,35-36(自科3):450-457.
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纳米羟基磷灰石携载lefty 1基因对人乳腺癌细胞的杀伤效应()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第35-36卷
期数:
2016年自科3期
页码:
450-457
栏目:
出版日期:
2016-05-10

文章信息/Info

Title:
Killing Effect of Nanohydroxyapatite Loading Lefty 1 Gene on Human Breast Carcinoma Cells
文章编号:
1673-3851 (2016) 03-0450-08
作者:
钟云平; 姚晨雪; 宋国龙; 赖文; 陈岑; 解纯刚; 孔祥东
浙江理工大学，a. 生命科学学院；b. 材料与纺织学院，杭州 310018
Author(s):
ZHONG Yunping;  YAO Chenxue;  SONG Guolong;  LAI Wen;  CHEN Cen;  XIE Chungang;  KONG Xiangdong
a. College of Life Sciences; b. College of Materials and Textile,  Zhejiang Sci-Tech University,Hangzhou 310018, China
关键词:
羟基磷灰石; lefty1; 人乳腺癌细胞; 杀伤效应; 聚乙烯亚胺
分类号:
Q279
文献标志码:
A
摘要:
采用聚乙烯亚胺为调控剂，氯化钙和磷酸氢二钠为原料，共沉淀法合成一种长梭状纳米羟基磷灰石。通过场发射扫描电镜、X射线粉末衍射、红外吸收光谱和热重分析仪对材料性能进行表征。体外降解实验表明羟基磷灰石颗粒具有可降解性，在弱酸性条件下更容易降解，具有pH值响应性。颗粒与质粒DNA体外负载曲线及基因释放曲线结果表明，长梭状羟基磷灰石颗粒能够有效地携载并且表现对质粒DNA的持续释放。体外转染实验证实颗粒携载pEGFPC1lefty1质粒后在细胞中成功表达绿色荧光蛋白。MTT结果表明所制备的羟基磷灰石颗粒对人乳腺上皮细胞表现极低的细胞毒性，具有良好的生物相容性，携载pEGFPC1lefty1质粒后对人乳腺癌细胞具有良好的杀伤效果。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期： 2015-12-23
基金项目： 国家自然科学基金项目(50942023，51272236)；浙江理工大学521人才培养计划资助项目（1610032521302）
作者简介： 钟云平(1990-)，男，浙江湖州人，硕士研究生，主要从事生物材料方面的研究
通信作者： 孔祥东，E-mail：kongxiangdong@gmail.com
更新日期/Last Update: 2016-06-06