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[1]孙笑竹,吕春伟,秦云,等.CDK抑制剂SNS 032联合AD55 Apoptin对肝癌细胞Huh 7的体外抗癌作用[J].浙江理工大学学报,2016,35-36(自科2):290-296.
　SUN Xiaozhu,L  Chunwei,QIN Yun,et al.In vitro Antitumor Effect of Combining CDK Inhibitor SNS 032  with AD55 Apoptin on Liver Carcinoma Cell Huh[J].Journal of Zhejiang Sci-Tech University,2016,35-36(自科2):290-296.
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CDK抑制剂SNS 032联合AD55 Apoptin对肝癌细胞Huh 7的体外抗癌作用()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第35-36卷
期数:
2016年自科2期
页码:
290-296
栏目:
出版日期:
2016-03-10

文章信息/Info

Title:
In vitro Antitumor Effect of Combining CDK Inhibitor SNS 032  with AD55 Apoptin on Liver Carcinoma Cell Huh
文章编号:
1673-3851 (2016) 02-0290-07
作者:
孙笑竹; 吕春伟; 秦云; 韩建翠; 王毅刚
浙江理工大学生命科学学院，杭州 310018
Author(s):
SUN Xiaozhu;  L  Chunwei;  QIN Yun;  HAN Jiancui;  WANG Yigang
College of Life Science, Zhejiang Sci-Tech University, Hangzhou 310018, China
关键词:
CDK抑制剂（SNS032）; AD55Apoptin; 肝癌; 体外; 抗癌作用
分类号:
Q26
文献标志码:
A
摘要:
通过CDK抑制剂SNS 032联合AD55 Apoptin处理肝癌Huh 7细胞，以探究两者联合作用的体外抗癌作用。采用MTT法分别检测SNS 032、AD55 Apoptin以及SNS 032与AD55 Apoptin联合对肝癌细胞株Huh7生长的抑制作用；利用Hoechst33342染色观察所处理细胞的凋亡形态学变化，采用流式细胞术对凋亡进行量化，Western Blot检测凋亡相关蛋白表达情况。结果表明：10MOI的AD55 Apoptin联合100ng/mL的SNS 032处理96h后，Huh 7细胞的存活率仅为4%，而10 MOI的AD55 Apoptin和100ng/mL SNS 032分别单独处理后细胞存活率为60%和8%（ P <0.05）；Hoechst33342染色、流式检测及Western Blot结果表明，联合处理组细胞凋亡现象更明显。实验结果证明SNS 032联合AD55Apoptin能有效的抑制肝癌细胞Huh 7生长，并诱导其凋亡。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期： 2015-04-28
基金项目： 浙江省自然科学基金项目(LY13H080005)；浙江省科技计划项目（2014C37101）；浙江理工大学科研启动基金项目(14040365J)
作者简介： 孙笑竹（1989-），女，辽宁锦州人，硕士研究生，主要从事癌症的靶向基因病毒疗法方面的研究
通信作者：王毅刚，E-mail：ygwang@zstu.edu.cn
更新日期/Last Update: 2016-06-02