|本期目录/Table of Contents|

[1]陈程,徐向群.桦褐孔菌子实体三萜单体的分离及鉴定[J].浙江理工大学学报,2015,33-34(自科2):264-268.
　CHEN Cheng,XU Xiang qun.Isolation and Identification of Triterpenoids in  Inonotus Obliquus[J].Journal of Zhejiang Sci-Tech University,2015,33-34(自科2):264-268.
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桦褐孔菌子实体三萜单体的分离及鉴定()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第33-34卷
期数:
2015年自科2期
页码:
264-268
栏目:
(自科)生物与生命科学
出版日期:
2015-03-10

文章信息/Info

Title:
Isolation and Identification of Triterpenoids in  Inonotus Obliquus
文章编号:
1673-3851 (2015) 02-0264-05
作者:
陈程;  徐向群
浙江理工大学理学院, 杭州 310018
Author(s):
CHEN Cheng;  XU Xiang qun
School of Science, Zhejiang Sci-Tech University, Hangzhou 310018, China
关键词:
桦褐孔菌;  硅胶柱色谱法;  三萜化合物;  羊毛甾醇;  栓菌酸
分类号:
O629
文献标志码:
A
摘要:
利用超声破壁处理,氯仿为溶剂对桦褐孔菌子实体中三萜单体进行提取。通过薄层层析色谱(TLC)检测,用1%香草醛浓硫酸试剂作为显色剂,反复用硅胶柱色谱对氯仿提取物进行梯度洗脱,得到两种羊毛脂烷型的三萜化合物,即化合物1(121 mg)和化合物2(67 mg)。采用HPLC的方法在206 nm处分析其纯度,同时红外光谱(IR)分析两种化合物的特征,以及核磁共振( 1HNMR和 13 C NMR)技术对两种羊毛脂烷型的三萜化合物进行结构鉴定。对比已知标准谱图,最终确定其结构为羊毛甾醇(Lanosterol)和栓菌酸(Trametenolic acid)。

参考文献/References:

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［8］ Bai Y H, Feng Y Q, Mao D B, et al. Optimization for betulin production from mycelial culture of Inonotus obliquus by orthogonal design and evaluation of its antioxidant activity［J］. Journal of the Taiwan Institute of Chemical Engineers, 2012, 43(5): 663-669.
［9］ Kahlos  K, Hiltunen R, v Schantz M. 3βHydroxylanosta8, 24dien21al, a new triterpene from Inontus obliquus［J］. Planta Medica, 1984, 50(2): 197-198.
［10］ Tamai  Y, Terazawa M. Chemical constituents of  Inonotus obliquus  I. A new triterpene, 3β hydroxy 8, 24 dien lanosta 21, 23 lactone from sclerotium［J］. Eurasian Journal of Forest Research, 2000, 1: 43-50.

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备注/Memo

备注/Memo:
收稿日期： 2014-03-17
基金项目： 浙江省科技厅科技计划项目(2012C23075)
作者简介： 陈程(1988-),女,浙江绍兴人,硕士研究生,主要从事生物技术与生物化工方面的研究
通信作者： 徐向群,E-mail:xuxiangqun@zstu.edu.cn
更新日期/Last Update: 2015-03-24