|本期目录/Table of Contents|

[1]付学忠,刘琳,姚菊明. 羊毛角蛋白的提取工艺及其特性表征[J].浙江理工大学学报,2012,29(02):160-163.
　FU Xue\|zhong,LIU Lin,YAO Ju\|ming. The Extraction and Characterization of Keratin from Wool[J].Journal of Zhejiang Sci-Tech University,2012,29(02):160-163.
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 羊毛角蛋白的提取工艺及其特性表征()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第29卷
期数:
2012年02期
页码:
160-163
栏目:
(自科)纺织与服装工程
出版日期:
2012-04-28

文章信息/Info

Title:
 The Extraction and Characterization of Keratin from Wool
文章编号:
16733851 (2012) 02016004
作者:
 付学忠;  刘琳;  姚菊明
 浙江理工大学先进纺织材料与制备技术教育部重点实验室， 杭州 310018
Author(s):
 FU Xue\|zhong;  LIU Lin;  YAO Ju\|ming
 The Key Laboratory of Advanced Textile Materials and Manufacturing Technology(Zhejiang Sci\|Tech University), Ministry of Education, Hangzhou 310018, China
关键词:
 ; 羊毛;  角蛋白;  十二烷基磺酸钠;  产率;  分子量;  二级结构
分类号:
TS102.31
文献标志码:
A
摘要:
    采用化学还原法从羊毛中提取角蛋白，制备角蛋白膜，并采用红外、电泳等测试方法对提取的角蛋白进行表征，同时探讨在透析液中加入少量十二烷基磺酸钠对角蛋白提取过程的影响。结果表明：提取角蛋白的分子量集中在50~70 kD；角蛋白在溶解过程中并没发生肽键的断裂，在透析时部分断裂的二硫键再次被氧化；透析液中加入十二烷基磺酸钠，可溶性角蛋白的产率由56%提高到78%，且十二烷基磺酸钠的加入对所提取角蛋白的分子量和内部结构没有明显影响。

参考文献/References:

 ［1］ Zoccola M, Aluigi A, Vineis C, et al. Study on cast membranes and electrospun nanofibers made from keratin/fibroin blends［J］. Biomacromolecules, 2008, 9(10): 2819\|2825
［2］ Salminen E, Rintala J. Anaerobic digestion of organic solid poultry slaughterhouse waste［J］. Bioresource Technology, 2002, 83: 13\|26.
［3］ Yin J, Rastogi S, Terry A E, et al. Self\|organization of oli\|gopeptides obtained on dissolution of feat her keratins in super\|heated water［J］. Biomacromolecules, 2007, 8 (3): 800\|806. 
［4］ Tanabe T, Okitsu N, Tachibana A, et al. Preparation and characterization of keratin\|chitosan composite film［J］. Biomaterials, 2002, 23(3): 817\|825.
［5］ Rhodes H J, Potter B, Widra A. Characteristics of the alpha\|keratose fraction of hair inducing ascosporogenesis in Nannizzia Grubyia［J］. Mycopathologia, 1967, 33(3): 345\|348.
［6］Thomas H, Conrads A, Phan K\|H, et al. In vitro reconstitution of wool intermediate filaments［J］. International Journal of Biological Macromolecules, 1986, 8(5): 258\|264.
［7］ Takeaki M, Hiroshi S, Hiroshi I. The phase transition in alpha\|keratin fibers［J］. Bulletin of the Institute for Chemical Research, 1987, 65(2): 109\|119. 
［8］ Yamauchi K, Yamauchi A, Kusunoki T, et al. Preparation of stable aqueous solution of keratins, and physiochemical and biodegradational properties of films［J］. Journal of Biomedical Materials Research, 1996, 31(4): 439\|444.
［9］ Katoh K, Tanabe T, Yamauchi K. Novel approach to fabricate keratin sponge scaffolds with controlled pore size and porosity［J］. Biomaterial, 2004, 25(18): 4255\|4262.
［10］ 蔡婷, 赵耀明, 杨崇玲. 羽毛角蛋白溶液的制备及研究［J］. 印染助剂, 2007, 24(6): 20\|23.
［11］ 熊小明, 胡仲禹. 废弃毛类物质的提取物：角蛋白的制备及应用［J］. 江西化工, 2007（3）: 8\|12.
［12］ Schrooyen P M M, Dijkstra P J, Oberthur R C, et al. Stabilization of solutions of feather keratins by sodium dodecyl sulfate［J］. Journal of Colloid and Interface Science, 2001, 240(1): 30\|39.
［13］ 李文霞, 廖青, 刘燚. 利用付立叶红外光谱与付立叶拉曼光谱初探超细羊毛粉的光谱行为［J］. 分析科学学报, 2007, 23(5): 519\|522.
［14］ 贾如琰, 何玉凤, 王荣民, 等. 角蛋白的分子构成、 提取及应用［J］. 化学通报, 2008（4）: 265\|271.
［15］ Jeanette M. Cardamone. Investigating the microstructure of keratin extracted from wool: peptide sequence (MALDI\|TOF/TOF) and protein conformation (FTIR)［J］. Journal of Molecular Structure, 2010, 969(1): 97\|105.

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备注/Memo

备注/Memo:
 收稿日期： 2011-09-06
基金项目： 教育部新世纪人才支持计划（NCET070763）
作者简介： 付学忠（1987-），男，山东德州人，硕士研究生，主要从事生物高分子材料的研究。
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