|本期目录/Table of Contents|

[1]刘芹,张秋桠,姜永厚.白斑综合征病毒PCR检测方法的建立及初步应用[J].浙江理工大学学报,2023,49-50(自科五):651-663.
 LIU Qin,ZHANG Qiuya,JIANG Yonghou.Establishment and preliminary application of a PCR  detection method for white spot syndrome virus[J].Journal of Zhejiang Sci-Tech University,2023,49-50(自科五):651-663.
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白斑综合征病毒PCR检测方法的建立及初步应用()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第49-50卷
期数:
2023年自科第五期
页码:
651-663
栏目:
出版日期:
2023-09-24

文章信息/Info

Title:
Establishment and preliminary application of a PCR  detection method for white spot syndrome virus
文章编号:
1673-3851 (2023) 09-0645-06
作者:
刘芹张秋桠姜永厚
浙江理工大学生命科学与医药学院,杭州 310018
Author(s):
LIU Qin ZHANG Qiuya JIANG Yonghou
College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China
关键词:
白斑综合征病毒检测PCR特异性灵敏度对虾
分类号:
S945-19;S945-4
文献标志码:
A
摘要:
引起白斑综合征(White spot syndrome, WSS)的白斑综合征病毒(White spot syndrome virus,WSSV)是一种传播速度快、致死率高的对虾病毒,对全球对虾养殖业构成了严重威胁。为及早诊断WSS,防止WSS的传播和暴发,有必要开发一种适用于对虾养殖场所的高灵敏度WSSV检测方法。该文针对保守区VP28序列设计和优化了一种可以特异地扩增WSSV的聚合酶链式反应(Polymerase chain reaction,PCR)检测方法。WSSV扩增产物在凝胶电泳图上可观察到清晰且单一的目的条带,而非靶标病毒则没有对应扩增产物出现,最低检测限可以达到50拷贝/反应;利用该PCR方法检测杭州地区采集的112份对虾混样样品,WSSV阳性率为15.7%。该特异和灵敏的PCR方法可被应用于WSS流行早期监控。

参考文献/References:

1 Jeeva S, Kim N I, Jang I K, et al. Development of a multiplex PCR system for the simultaneous detection of white spot syndrome virus and hepatopancreatic parvovirus infectionJ. Aquaculture Research, 2014, 45(6): 1073-1083.

2Qiu W, Geng R, Zuo H L, et al. Toll receptor 2 (Toll2) positively regulates antibacterial immunity but promotes white spot syndrome virus (WSSV) infection in shrimpJ. Developmental & Comparative Immunology, 2021, 115: 103878.

3Verbruggen B, Bickley L K, van Aerle R, et al. Molecular mechanisms of white spot syndrome virus infection and perspectives on treatmentsJ. Viruses, 2016, 8(1): 23.

4Zhu G H, Li S H, Wu J, et al. Identification of functional gene modules associated with STAT-mediated antiviral responses to white spot syndrome virus in shrimpJ. Frontiers in Physiology, 2019, 10: 212.

5Lightner D V, Redman R M, Pantoja C R, et al. Historic emergence, impact and current status of shrimp pathogens in the AmericasJ. Journal of Invertebrate Pathology, 2012, 110(2):174-183.

6Stentiford G D, Neil D M, Peeler E J, et al. Disease will limit future food supply from the global crustacean fishery and aquaculture sectorsJ. Journal of Invertebrate Pathology, 2012, 110(2): 141-157.

7Xu J D, Jiang H S, Wei T D, et al. Interaction of the small GTPase Cdc42 with arginine kinase restricts white spot syndrome virus in shrimpJ. Journal of Virology, 2017, 91(5): e01916.

8Nunan L M, Lightner D V. Development of a non-radioactive gene probe by PCR for detection of white spot syndrome virus (WSSV)J. Journal of Virological Methods, 1997, 63(1/2): 193-201.

9Zhan W B, Wang Y H, Fryer J L, et al. Production of monoclonal antibodies (MAbs) against white spot syndrome virus (WSSV)J. Journal of Aquatic Animal Health, 1999, 11(1): 17-22.

10Cheng Q Y, Meng X L, Xu J P, et al. Development of lateral-flow immunoassay for WSSV with polyclonal antibodies raised against recombinant VP (19+28) fusion proteinJ. Virologica Sinica, 2007, 22(1): 61-67.

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备注/Memo

备注/Memo:
收稿日期: 2023-05-21
网络出版日期:2023-07-07
基金项目: 浙江省自然科学基金项目 (LGC21C180002)
作者简介: 刘芹(1998—),女,安徽滁州人,硕士研究生,主要从事病毒分子诊断方面的研究
通信作者: 姜永厚,E-mail: yonghoujiang@zstu.edu.cn
更新日期/Last Update: 2023-09-19