|本期目录/Table of Contents|

[1]邓明,夏冠赫,刘锋,等.负载生长因子水凝胶的体外生物活性研究[J].浙江理工大学学报,2023,49-50(自科五):581-588.
 DENG Ming,XIA Guanhe,LIU Feng,et al.Study on the  in vitro  bioactivity of hydrogels  loaded with growth factors[J].Journal of Zhejiang Sci-Tech University,2023,49-50(自科五):581-588.
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负载生长因子水凝胶的体外生物活性研究()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第49-50卷
期数:
2023年自科第五期
页码:
581-588
栏目:
出版日期:
2023-09-24

文章信息/Info

Title:
Study on the  in vitro  bioactivity of hydrogels  loaded with growth factors
文章编号:
1673-3851 (2023) 09-0581-08
作者:
邓明夏冠赫刘锋李佳乐王秉
浙江理工大学材料科学与工程学院,杭州 310018
Author(s):
DENG Ming XIA Guanhe LIU Feng LI Jiale WANG Bing
School of Materials Science & Engineering, Zhejiang Sci-Tech University, Hangzhou 310018, China
关键词:
生长因子丝素蛋白明胶水凝胶促胶原蛋白合成
分类号:
TB-34
文献标志码:
A
摘要:
为了开发一种新型生物活性伤口敷料,以丝素蛋白(SF)和酪胺改性明胶(TG)为基体材料,通过辣根过氧化物酶和过氧化氢催化交联制备三维网状水凝胶,并用水凝胶负载碱性成纤维细胞生长因子(bFGF),制备SF TG bFGF 水凝胶。对SF TG bFGF进行结构分析和形貌表征,检测SF TG bFGF对小鼠成纤维细胞L929增殖、迁移和胶原蛋白合成的影响。结果表明:SF TG bFGF水凝胶具有良好的力学性能、吸湿保湿性能和生长因子缓释性能;SF TG bFGF呈网状微观结构,弹性模量为21 kPa,溶胀率高达700%,能在144 h内缓释bFGF;SF TG bFGF能促进L929细胞的增殖、迁移和胶原蛋白合成,细胞存活率可达300%,细胞迁移率可达90%,胶原蛋白质量浓度可达4.5 μg/mL。该研究制备的SF TG bFGF有望作为一种生物活性敷料应用于各类皮肤伤口治疗。

参考文献/References:

1 Liang Y P, He J H, Guo B L. Functional hydrogels as wound dressing to enhance wound healingJ. ACS Nano, 2021, 15(8): 12687-12722.

2Kharaziha M, Baidya A, Annabi N. Rational design of immunomodulatory hydrogels for chronic wound healingJ. Advanced Materials, 2021, 33(39): e2100176.

3Gong X Y, Luo M, Wang M, et al. Injectable self-healing ceria-based nanocomposite hydrogel with ROS-scavenging activity for skin wound repairJ. Regenerative Biomaterials, 2021, 9: rbab074.

4Sivaraj D, Chen K, Chattopadhyay A, et al. Hydrogel scaffolds to deliver cell therapies for wound healingJ. Frontiers in Bioengineering and Biotechnology, 2021, 9: 660145.

5Asadi N, Pazoki-Toroudi H, Del Bakhshayesh A R, et al. Multifunctional hydrogels for wound healing: special focus on biomacromolecular based hydrogelsJ. International Journal of Biological Macromolecules, 2021, 170: 728-750.

6]董鸿斐, 黄启林, 杨熊, . 真皮细胞外基质水凝胶促进大鼠急性皮肤创面修复[J. 中国组织工程研究, 2022, 26(10): 1555-1560.

7Zhao X, Sun X M, Yildirimer L, et al. Cell infiltrative hydrogel fibrous scaffolds for accelerated wound healingJ. Acta Biomaterialia, 2017, 49: 66-77.

8]姚晶, 徐国平, 王悦, . 聚乳酸/壳聚糖/明胶复合丝线的性能分析[J. 浙江理工大学学报(自然科学版), 2022, 47(2): 159-165.

9Qiu W W, Han H, Li M, et al. Nanofibers reinforced injectable hydrogel with self-healing, antibacterial, and hemostatic properties for chronic wound healingJ. Journal of Colloid and Interface Science, 2021, 596: 312-323.

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备注/Memo

备注/Memo:
收稿日期: 2023-01-07
网络出版日期:2023-05-05
基金项目: 国家自然科学基金项目(52273096);浙江省自然科学基金项目(LQ15E030004)
作者简介: 邓明(1999-),男,湖南衡阳人,硕士研究生,主要从事生物材料方面的研究
通信作者: 王秉,E-mail:wbing388@163.com
更新日期/Last Update: 2023-09-19