|本期目录/Table of Contents|

[1]杨旭,陈岑,丁亚辉.磷灰石/钛基涂层表面携载高黄芩苷/低栀子苷配伍的协同促内皮抗增殖作用[J].浙江理工大学学报,2022,47-48(自科一):123-130.
　YANG Xu,CHEN Cen,DING Yahui.The endothelial promotion and anti proliferation effects of the  combination of high baicalin/low geniposide on apatite/titanium based coating surface[J].Journal of Zhejiang Sci-Tech University,2022,47-48(自科一):123-130.
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磷灰石/钛基涂层表面携载高黄芩苷/低栀子苷配伍的协同促内皮抗增殖作用()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第47-48卷
期数:
2022年自科第一期
页码:
123-130
栏目:
出版日期:
2022-01-31

文章信息/Info

Title:
The endothelial promotion and anti proliferation effects of the  combination of high baicalin/low geniposide on apatite/titanium based coating surface
文章编号:
1673-3851 (2022) 01-0123-08
作者:
杨旭; 陈岑; 丁亚辉
1.浙江理工大学生命科学与医药学院，杭州310018；2.杭州医学院附属人民医院，杭州310014；3.浙江省人民医院心内科，杭州 310014
Author(s):
YANG Xu;  CHEN Cen;  DING Yahui
1.College of Life Sciences and Medicine, Zhejiang SciTechUniversity, Hangzhou 310018, China; 2.People′s Hospital of Hangzhou Medical College, Hangzhou 310014, China; 3.Department of  Cardiology, Zhejiang Provincial People’s Hospital, Hangzhou 310014, China
关键词:
栀子苷; 黄芩苷; 磷灰石; 配伍载药; 平滑肌细胞; 内皮细胞; 药物洗脱支架
分类号:
Q28
文献标志码:
A
摘要:
栀子苷、黄芩苷具有抗血栓、抗炎等药用价值，磷灰石涂层作为药物洗脱支架涂层具有抑制平滑肌细胞（smooth muscle cells，SMCs）增殖能力。为探究磷灰石药物洗脱支架涂层携载黄芩苷/栀子苷对SMCs增殖与内皮细胞（endothelial cells，ECs）生长的作用，将SMCs和ECs分别培养于含50、25 mg/L和125 mg/L黄芩苷或栀子苷的培养基中，观察细胞活性，筛选有效作用浓度；在磷灰石涂层表面分别携载10、100 μg和150 μg栀子苷或黄芩苷，并将细胞接种于涂层表面，观察细胞活性；将150 μg黄芩苷/10 μg栀子苷配伍、150 μg栀子苷/10 μg黄芩苷配伍载于磷灰石涂层表面，通过CCK8、细胞骨架/细胞核染色检测并观察细胞活性及细胞形貌。结果表明：黄芩苷、栀子苷均能在前期抑制SMCs活性并促进ECs增殖。150 μg黄芩苷/磷灰石涂层对SMCs的细胞活性有明显的抑制作用，10 μg栀子苷/磷灰石涂层相较于100 μg和150 μg对SMCs的活性影响更大，而对ECs无明显作用；配伍实验显示150 μg黄芩苷/10 μg栀子苷可显著降低SMCs活性，且对ECs作用较小。因此，高黄芩苷/低栀子苷配伍的磷灰石洗脱涂层有望应用于新一代药物洗脱支架的研究与开发。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期： 2021-09-03
网络出版日期：2021-09-18
基金项目：浙江省中医药管理局计划项目（2018ZB011）
作者简介：杨旭(1998-)，女，江苏省扬州人，本科生，主要从事心血管支架方面的研究
通信作者：丁亚辉，E-mail：doctordyh@163.com
更新日期/Last Update: 2022-03-08