|本期目录/Table of Contents|

[1]高双玉,杜志游.关于二羧酸-三羧酸盐载体蛋白负调控黄瓜花叶病毒积累的初步研究[J].浙江理工大学学报,2018,39-40(自科5):613-618.
 GAO Shuangyu,DU Zhiyou.Preliminary study on the negative regulation of  Cucumber mosaic virus accumulation by dicarboxylatetricarboxylate carrier[J].Journal of Zhejiang Sci-Tech University,2018,39-40(自科5):613-618.
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关于二羧酸-三羧酸盐载体蛋白负调控黄瓜花叶病毒积累的初步研究()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第39-40卷
期数:
2018年自科5期
页码:
613-618
栏目:
出版日期:
2018-08-31

文章信息/Info

Title:
Preliminary study on the negative regulation of  Cucumber mosaic virus accumulation by dicarboxylatetricarboxylate carrier
文章编号:
1673-3851 (2018) 09-0613-06
作者:
高双玉杜志游
浙江理工大学生命科学学院,杭州 310018
Author(s):
GAO Shuangyu DU Zhiyou
College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China
关键词:
黄瓜花叶病毒复制二羧酸三羧酸盐载体蛋白
分类号:
Q933
文献标志码:
A
摘要:
黄瓜花叶病毒(Cucumber mosaic virus,CMV)的复制场所位于液泡膜,但是参与调控CMV复制的寄主蛋白因子有待进一步研究。植物二羧酸三羧酸盐载体蛋白(Dicarboxylatetricarboxylate carrier,DTC)为线粒体膜和液泡膜定位的跨膜蛋白,参与二羧酸盐和三羧酸盐的跨膜转运。为了探索DTC在CMV复制中的调控作用,通过基于烟草脆裂病毒( Tobacco rattle virus, TRV)的基因载体下调本氏烟体内DTC-2(NbDTC2)的mRNA水平,并通过GFP荧光和Western blot分析DTC2下调表达后对CMVΔ2beGFP积累量的影响,发现NbDTC2的下调表达增加CMV的积累水平;同时分析本氏烟DTC2的瞬时过表达对CMVΔCP eGFP积累的影响,通过观察eGFP荧光和Western blot检测,发现NbDTC2的过表达在一定程度上抑制病毒的积累。以上结果表明:DTC2负调控CMV的积累,可能参与植物对病毒的防御。

参考文献/References:

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[3] Mochizuki T, Ohki S T. Cucumber mosaic virus: Viral genes as virulence determinants[J]. Molecular Plant Pathology,2012,13(3):217-225.
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[5] Perret V, Florentz C, Dreher T, et al. Structural analogies between the 3’ tRNAlike structure of Brome mosaic virus RNA and yeast tRNATyr revealed by protection studies with yeast tyrosyltRNA synthetase[J]. European Journal of Biochemistry,1989,185(2):331-339.
[6] Zhang X P, Liu D S, Yan T et al. Cucumber mosaic virus coat protein modulates the accumulation of 2b protein and antiviral silencing that causes symptom recovery in planta[J]. PLoS Pathogen,2017,13(7):e1006522.
[7] Du Z Y, Chen A Z, Chen W H, et al. Nuclearcytoplasmic partitioning of Cucumber mosaic virus protein 2b determines the balance between its roles as a virulence determinant and an RNAsilencing suppressor[J]. Journal of Virology,2014,88(10),5228-5241.
[8] Cillo F, I M R, Palukaitis P. In situ localization and tissue distribution of the replicationassociated proteins of Cucumber mosaic virus in tobacco and cucumber[J]. Journal of Virology,2002,76(21):10654-10664.
[9] Mise K, Ahlquist P. Hostspecificity restriction by Bromovirus celltocell movement protein occurs after initial celltocell spread of infection in nonhost plants[J]. Virology,1995,206(1):276-286.
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相似文献/References:

[1]卢冉,常发光,杜志游,等.以黄瓜花叶病毒为载体表达绿色荧光蛋白[J].浙江理工大学学报,2016,35-36(自科6):917.
 LU Ran,CHANG Faguang,DU Zhiyou,et al.Green Fluorescent Protein Expression Based on Cucumber Mosaic Virus[J].Journal of Zhejiang Sci-Tech University,2016,35-36(自科5):917.
[2]朱品,常发光,杜志游,等.基于黄瓜花叶病毒基因组RNA2的外源基因表达载体研究[J].浙江理工大学学报,2017,37-38(自科2):265.
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备注/Memo

备注/Memo:
收稿日期: 2018-05-11
网络出版日期: 2018-05-30
基金项目: 国家自然科学基金项目(31470007)
作者简介: 高双玉(1993- ),女,安徽阜阳,硕士研究生,主要从事植物病理方面的研究
通信作者: 杜志游,E-mail:duzy@zstu.edu.cn
更新日期/Last Update: 2018-09-12