|本期目录/Table of Contents|

[1]罗丹丹,崔正阳,冯翠,等.磷灰石涂层用于调控神经细胞的生物学行为[J].浙江理工大学学报,2018,39-40(自科4):484-490.
 LUO Dandan,CUI Zhengyang,FENG Cui,et al.Biological behavior of apatite coating used to regulate nerve cell[J].Journal of Zhejiang Sci-Tech University,2018,39-40(自科4):484-490.
点击复制

磷灰石涂层用于调控神经细胞的生物学行为()
分享到:

浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第39-40卷
期数:
2018年自科4期
页码:
484-490
栏目:
出版日期:
2018-07-10

文章信息/Info

Title:
Biological behavior of apatite coating used to regulate nerve cell
文章编号:
1673-3851 (2018) 07-0484-07
作者:
罗丹丹崔正阳冯翠马矢徒孔祥东
浙江理工大学,a.生命科学学院;b.材料与纺织学院,杭州 310018
Author(s):
LUO Dandan CUI Zhengyang FENG Cui MA Shitu KONG Xiangdong
a.College of Life Science; b.College of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou 310018, China
关键词:
磷灰石层粘连蛋白释放PC12细胞
分类号:
TS195.644
文献标志码:
A
摘要:
利用仿生矿化法在钛合金表面制备磷灰石(Apatite)涂层,并通过仿生共沉积的方式在该涂层表面固定层粘连蛋白(Laminin,LN),在钛合金表面构建磷灰石LN功能性复合涂层。通过场发射扫描电子显微镜(FESEM)和X射线光电子能谱仪(XPS)对磷灰石与磷灰石LN复合涂层进行表征,发现制备的磷灰石涂层呈现均一的多孔片层状结构,对LN分子有良好的固定能力。磷灰石LN复合涂层的LN释放实验结果表明:构建的磷灰石LN复合涂层中的LN分子可从涂层表面缓慢而持续地释放。神经细胞PC12与磷灰石、磷灰石LN复合涂层的联合体外培养实验结果表明:磷灰石、磷灰石LN复合涂层具有良好的生物相容性,且磷灰石LN复合涂层具有一定的促进神经细胞贴附和增殖的作用。制备的磷灰石涂层作为仿生共沉积生物活性分子的理想载体,在调控神经细胞生长以及周围神经损伤的修复领域有着良好的应用前景。

参考文献/References:

[1] Li R, Liu Z, Pan Y, et al. Peripheral nerve injuries treatment: A systematic review[J]. Cell Biochemistry & Biophysics,2014,68(3):449-454.
[2] Zochodne D W. The microenvironment of injured and regenerating peripheral nerves[J]. Muscle & Nerve,2015,23(S9):S33-S38.
[3] Gu X, Ding F, Williams D F. Neural tissue engineering 〖JP2〗options for peripheral nerve regeneration[J]. Biomaterials,2014,35(24):6143-6156.
[4] Neal R A, Tholpady S S, Foley P L, et al. Alignment and composition of lamininpolycaprolactone nanofiber blends enhance peripheral nerve regeneration[J]. Journal of Biomedical Materials Research Part A,2011,100A(2):406-423.
[5] Li A, Hokugo A, Yalom A, et al. A bioengineered peripheral nerve construct using aligned peptide amphiphile nanofibers[J]. Biomaterials,2014,35(31):8780-8790.
[6] Costa F M, Maia S R, Gomes P A, et al. Dhvar5 antimicrobial peptide (AMP) chemoselective covalent immobilization results on higher antiadherence effect than simple physical adsorption[J]. Biomaterials,2015,52(1):531-538.
[7] Holt A P, Bocharova V, Cheng S, et al. Controlling interfacial dynamics: Covalent bonding versus physical adsorption in polymer nanocomposites[J]. Acs Nano,2016,10(7):6843-6852.
[8] Ong J L, Bess E G, Bessho K. Osteoblast progenitor cell responses to characterized titanium surfaces in the presence of bone morphogenetic proteinatelopeptide type I collagen in vitro[J]. Journal of Oral Implantology,1999,25(2):95-100.
[9] Yang K, Lee J S, Kim J, et al. Polydopaminemediated surface modification of scaffold materials for human neural stem cell engineering[J]. Biomaterials,2012,33(29):6952-6964.
[10] Gagner J E, Qian X, Lopez M M, et al. Effect of gold nanoparticle structure on the conformation and function of adsorbed proteins[J]. Biomaterials,2012,33(33):8503-8516.

备注/Memo

备注/Memo:
收稿日期: 2018-04-08
网络出版日期: 2018-06-02
作者简介: 罗丹丹(1992-),女,安徽青阳人,硕士研究生,主要从事生物材料方面的研究
通信作者: 孔祥东,E-mail:kxd01@126.com
更新日期/Last Update: 2018-09-10