|本期目录/Table of Contents|

[1]郝亚,白雪,阮世超,等.维生素C对内皮细胞和平滑肌细胞增殖的影响[J].浙江理工大学学报,2018,39-40(自科3):352-356.
 HAO Ya,BAI Xue,RUAN Shichao,et al.Effects of vitamin C on proliferation of endothelial cells and smooth muscle cells[J].Journal of Zhejiang Sci-Tech University,2018,39-40(自科3):352-356.
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维生素C对内皮细胞和平滑肌细胞增殖的影响()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第39-40卷
期数:
2018年自科3期
页码:
352-356
栏目:
出版日期:
2018-05-19

文章信息/Info

Title:
Effects of vitamin C on proliferation of endothelial cells and smooth muscle cells
文章编号:
1673-3851 (2018) 05-0352-05
作者:
郝亚白雪阮世超庄青叶陈岑
浙江理工大学生命科学学院,杭州 310018
Author(s):
HAO Ya BAI Xue RUAN Shichao ZHUANG Qingye CHEN Cen
College of Life Science, Zhejiang Sci-Tech University, Hangzhou 310018, China
关键词:
药物洗脱支架内皮细胞平滑肌细胞维生素C雷帕霉素
分类号:
Q28
文献标志码:
A
摘要:
药物洗脱支架(Drugeluting stent, DES)释放抗增殖的药物来抑制平滑肌细胞(Smooth muscle cells, SMCs)增殖,阻止内膜增生。但这些药物不可避免地会延迟内皮化,因此有必要寻找合适的药物选择性的抑制SMC增殖,同时促进内皮细胞(Endothelial cells, ECs)增殖。维生素C(Lascorbic acid, LAA)是一种用于口服或是直接加入细胞培养的药物,因此实验中在培养过夜的EC和SMC细胞中加入浓度为300 μg/mL的LAA、浓度为100 μg/mL 的雷帕霉素(Sirolimus,SIR)、高糖培养基(Dulbeccos modified eagle medium, DMEM)、杜氏磷酸缓冲液(Dulbeccos phosphate buffered  saline, DPBS)和乙醇各100 μL,培养7 d观察,结果表明:SIR能够抑制EC增殖,而LAA能够显著促进EC的增殖,同时也能够抑制SMC的生长;在培养过夜的EC和SMC细胞中加入100 μL不同浓度的LAA(1、100、300、500 μg/mL和1000 μg/mL),培养7 d后,1、100 μg/mL和300 μg/mL的LAA有利于EC增殖,而500 μg/mL和1000 μg/mL的LAA会抑制EC增殖,并且300 μg/mL和500 μg/mL的LAA可以显著抑制SMC增殖。由此表明维生素C可以成为潜在的用于药物洗脱支架的药物。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期: 2017-9-30
网络出版日期: 2017-12-29
基金项目: 国家自然科学基金项目(51502265);浙江省自然科学基金项目(LQ16E020006)
作者简介: 郝亚(1993-),女,甘肃酒泉人,硕士研究生,主要从事生物材料方面的研究
通信作者: 陈岑,E-mail:chencen313@gmail.com
更新日期/Last Update: 2018-06-19