|本期目录/Table of Contents|

[1]尤红,普倩,文芳,等.ACC脱氨酶菌株的分离筛选及对丹参毛状根的影响[J].浙江理工大学学报,2017,37-38(自科5):720-726.
 YOU Hong,PU Qian,WEN Fang,et al.Isolation and Screening of Bacteria Strain with ACC Deaminase Activity and Its Effect on Hairy Root of Salvia Miltiorrhiza[J].Journal of Zhejiang Sci-Tech University,2017,37-38(自科5):720-726.
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ACC脱氨酶菌株的分离筛选及对丹参毛状根的影响()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第37-38卷
期数:
2017年自科5期
页码:
720-726
栏目:
出版日期:
2017-08-30

文章信息/Info

Title:
Isolation and Screening of Bacteria Strain with ACC Deaminase Activity and Its Effect on Hairy Root of Salvia Miltiorrhiza
文章编号:
1673-3851 (2017) 05-0720-07
作者:
尤红普倩文芳饶君凤李欧胡秀芳
1.浙江理工大学,a.生命科学学院;b.浙江省植物次生代谢与调控重点实验室,杭州 310018;2.杭州职业技术学院临江学院,杭州 310018
Author(s):
YOU Hong PU Qian WEN Fang RAO Junfeng LI Ou HU Xiufang
1a.College of Life Science; 1b.Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation, Zhejiang SciTech University, Hangzhou 310018, China; 2.Linjiang School, Hangzhou Vocational and Technical College, Hangzhou 310018, China
关键词:
ACC脱氨酶生物固氮铁载体植物激素促生作用丹参毛状根丹酚酸假单胞菌
分类号:
Q939.96
文献标志码:
A
摘要:
为研究1氨基环丙烷1羧酸(1Aminocylopropane1carboxylic acid,ACC)脱氨酶菌株对丹参次生代谢物合成的影响,使用筛选获得的ACC脱氨酶活性最强的丹参根际菌株对丹参毛状根进行诱导,并分析诱导前后毛状根生物量以及药用活性物质含量的差异。结果表明:获得了ACC脱氨酶活性最高的菌株为DS3T3,其酶活力达到0.3899 U/mg,经鉴定该菌株为假单胞菌属细菌(Pseudomonas-sp.);丹参毛状根经该菌株诱导处理后,其干重与对照组相比增加16.22%,主要酚酸物质总量增加16.07%;对DS3T3进行进一步的测定结果表明,该菌株还具有固氮、产铁载体及产吲哚3乙酸和水杨酸等植物激素的能力。因此,该菌株可作为提高丹参产量以及品质的候选菌株。

参考文献/References:

[1] LIU L, YANG D, LIANG T, et al. Phosphate starvation promoted the accumulation of phenolic acids by inducing the key enzyme genes in Salvia miltiorrhiza hairy roots[J]. Plant Cell Reports,2016,35(9):1933-1942.
[2] 黄志成,任加惠,沈丽红,等.丹参毛状根和丹参药材HPLO指纹图谱的比较研究[J].浙江理工大学学报,2016,35(3):444-449.
[3] QIU D, SONG J. Danshens in Vitro Culture[M]. Berlin: Springer Netherlands,2015:69-87.
[4] SZYMA N′SKA S, PL′OCINICZAK T, PIOTROWSKASEGET Z, et al. Metabolic potential and community structure of endophytic and rhizosphere bacteria associated with the roots of the halophyte Aster tripolium L[J]. Microbiological Research,2016,182(2):68-79.
[5] ZHOU L G, WU J Y. Development and application of medicinal plant tissue cultures for production of drugs and herbal medicinals in China[J]. Natural Product Reports,2006,23(5):789-810.
[6] GLICK B R. Bacteria with ACC deaminase can promote plant growth and help to feed the world[J]. Microbiological Research,2014,169(1):30-39.
[7] XU M S, SHENG J P, CHEN L, et al. Bacterial community compositions of tomato (Lycopersicum esculentum Mill.) seeds and plant growth promoting activity of ACC deaminase producing Bacillus subtilis (HYT-12-1) on tomato seedlings[J]. World Journal of Microbiology and Biotechnology,2014,30(3):835-845.

备注/Memo

备注/Memo:
收稿日期: 2017-03-20
网络出版日期: 2017-08-07
基金项目: 浙江省公益农业项目(2015C32104);浙江省自然科学基金项目(LY15C010005);浙江理工大学启动基金项目(14042217-Y)
作者简介: 尤红(1990-),女,浙江嘉兴人,硕士研究生,主要从事药用植物次生代谢调控方面的研究
通信作者: 胡秀芳,E-mail:huxiuf@zstu.edu.cn
更新日期/Last Update: 2017-09-26