|本期目录/Table of Contents|

[1]邹胜利,聂作明,吴祥甫,等.水泡性口炎病毒与Monocyte及MoDC的互作研究[J].浙江理工大学学报,2016,35-36(自科5):749-753.
　ZOU Shengli,NIE Zuoming,WU Xiangfu,et al.Study on Interactions of Vesicular Stomatitis Virus with Monocyte and MoDC[J].Journal of Zhejiang Sci-Tech University,2016,35-36(自科5):749-753.
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水泡性口炎病毒与Monocyte及MoDC的互作研究()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第35-36卷
期数:
2016年自科5期
页码:
749-753
栏目:
出版日期:
2016-09-10

文章信息/Info

Title:
Study on Interactions of Vesicular Stomatitis Virus with Monocyte and MoDC
文章编号:
1673-3851 (2016) 05-0749-05
作者:
邹胜利; 聂作明; 吴祥甫; 方心葵; 孙涛
 1.浙江理工大学生物化学研究所，杭州 310018；2.上海交通大学上海市兽医生物技术重点实验室，上海 200240
Author(s):
ZOU Shengli;  NIE Zuoming;  WU Xiangfu;  Fang Xinkui;  SUN Tao
1.School of Life Science, Zhejiang Sci-Tech University, Hangzhou 310018, China; 2. Shanghai  Municipal Veterinary Key Laboratory, Shanghai Jiao Tong University, Shanghai 200240, China
关键词:
水泡性口炎病毒; 单核细胞; MoDC; 互作; 基质蛋白
分类号:
Q255
文献标志码:
A
摘要:
为了探究水泡性口炎病毒（Vesicular stomatitis virus ,VSV）与天然宿主免疫细胞的互作关系，实验用M蛋白第51位甲硫氨酸残基缺失后的重组病毒（VSVΔM51GFP）和第51位甲硫氨酸敲除、第221位缬氨酸突变为苯丙氨酸、第226位丝基酸突变为精氨酸的重组病毒（VSV M T GFP）以及野生型重组病毒（VSV GFP）感染猪单核细胞（Monocyte）和单核细胞诱导的树突状细胞（Monocytederived dendritic cell，MoDC），通过空斑实验制作病毒生长曲线，用激光共聚焦显微镜和流式细胞仪检测细胞的感染状况，ELISA检测细胞培养上清中的细胞因子表达变化。结果表明，VSV、VSVΔM51、VSVMT均可感染Monocyte和MoDC，且Monocyte的感染率仅为8.38%。3种病毒在MoDC中可以大量复制，但无法在Monocyte中扩增。且与野生型VSV相比，VSVΔM51、VSVMT细胞致病性依次减弱。VSV能有效激活TNFα、IL8、IFNα相关免疫细胞因子在MoDC中的表达，且M蛋白突变程度越大，病毒激活能力越强。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期： 2015-01-29
基金项目： 国家自然科学基金项目（31272562）
作者简介： 邹胜利（1990-），男，河南驻马店人，硕士研究生，主要从事分子生物学方面的研究
更新日期/Last Update: 2016-10-12