|本期目录/Table of Contents|

[1]吴程雨,叶飘,杨孝婷,等.固定化地衣芽孢杆菌胞外脂肪酶的制备[J].浙江理工大学学报,2015,33-34(自科5):694-698.
 WU Cheng yu,YE Piao,YANG Xiao ting,et al.Preparation of Immobilized Extracellular Lipase from Bacillus Licheniformis[J].Journal of Zhejiang Sci-Tech University,2015,33-34(自科5):694-698.
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固定化地衣芽孢杆菌胞外脂肪酶的制备()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第33-34卷
期数:
2015年自科5期
页码:
694-698
栏目:
出版日期:
2015-09-10

文章信息/Info

Title:
Preparation of Immobilized Extracellular Lipase from Bacillus Licheniformis
文章编号:
1673-3851 (2015) 05-0694-05
作者:
吴程雨 叶飘 杨孝婷 徐叶倩 巩孝磊 唐琦清 赵辅昆 潘建义 陈玮
浙江理工大学生命科学学院, 杭州 310018
Author(s):
WU Chengyu YE Piao YANG Xiaoting XU Yeqian GONG Xiaolei TANG QiqingZHAO Fukun PAN Jianyi CHEN Wei
School of Life Science, Zhejiang Science and Technology University, Hangzhou 310018, China
关键词:
地衣芽孢杆菌 脂肪酶 固定化 ZHHA
分类号:
Q786
文献标志码:
A
摘要:
脂肪酶是一类重要的水解酶,广泛应用于食品、医药、化工等领域。脂肪酶通过固定化可以提高酶的使用效率。文章采用戊二醛共价连接法将来源于地衣芽孢杆菌(Bacillus licheniformis)的胞外脂肪酶固定于氨基型载体ZHHA之上,将1 g载体置入10 mL酶液中反应3 h后固定化酶活力达到最高为60 U/g湿载体。经固定化后该酶的温度稳定性得到显著提升,当反应温度为45℃时最高酶活力达到220 U/g湿载体。同时,固定化使酶在碱性条件下的稳定性得到提高,最适反应pH值为10。通过自制柱式反应器考察该酶工作稳定性,经过15批连续水解反应,固定化脂肪酶仍保持90%的活力,展现出良好的稳定性。

参考文献/References:

[1] Treichel H, Oliveira D D, Mazutti M A, et al. A  review on microbial lipases production[J]. Food Bioprocess Technol, 2010, 3(2): 182-196.
[2] Ramani K, Boopathy R, Vidya C, et al. Immobilisation of pseudomonas gessardii acidic lipase derived from beef  tallow onto mesoporous activated carbon and its application on hydrolysis of olive oil[J]. Process Biochemistry, 2010, 45(6): 986-992.
[3] Dong H P, Wang Y J, Zheng Y G. Enantioselective hydrolysis of diethyl 3hydroxyglutarate to ethyl (s)3hydroxyglutarate by immobilized candida antarctica lipase B[J]. Journal of Molecular Catalysis B: Enzymatic, 2010, 66(1-2): 90-94.
[4] Izah S C, Ohimai E I. The challenge of biodiesel production from oil palm feedstock in nigeria[J]. Greener Journal of Biological Sciences, 2013, 3(1): 001-012.
[5] Speranza P, Macedo G A. Lipasemediated production  of specific lipids with improved biological and physicochemical properties[J]. Process Biochemistry, 2012, 47(12): 1699-1706.
[6] Atabani A E, Silitonga A S, Badruddin I A, et al. A  comprehensive review on biodiesel as an alternative energy resource and its characteristics[J]. Renewable and Sustainable Energy Reviews, 2012, 16(4): 2070-2093.
[7] Chisti Y. Biodiesel from microalgae[J]. Biotechnol Adv, 2007, 25(3): 294-306.
[8] RoblesMedina A, GonzalezMoreno P A, EstebanCerdan L, et al. Biocatalysis: towards ever greener biodiesel production[J]. Biotechnol Adv, 2009, 27(4): 398-408.
[9] Yusuf N N A N, Kamarudin S K, Yaakub Z. Overview on the current trends in biodiesel production[J]. Energy Conversion and Management, 2011, 52(7): 2741-2751.
[10] Balat  M. Potential alternatives to edible oils for biodiesel productiona review of current work[J]. Energy Conversion and Management, 2011, 52(2): 1479-1492.
[11] Holmquist  M. Alpha/betahydrolase fold enzymes: Structures, functions and mechanisms[J]. Current Protein & Peptide Science, 2000, 1(2): 209-235.
[12] Kallenberg  A I, Rantwijk F V, Sheldon R A. Immobilization of penicillin G acylase: the key to optimum performance[J]. Adv Synth Catal, 2005, 347(7-8): 905-926.
[13] 孙健, 周毅频, 陶家权, 等. 巨大芽孢杆菌青霉素G酰化酶在新型环氧载体ZHHA上的固定化[J]. 工业微生物, 2008, 38(1): 1-5.

相似文献/References:

[1]吴程雨,叶飘,杨孝婷,等.固定化地衣芽孢杆菌胞外脂肪酶的制备[J].浙江理工大学学报,2015,33-34(自科4):543.
 WU Cheng yu,YE Piao,YANG Xiao ting,et al.Preparation of Immobilized Extracellular Lipase from Bacillus Licheniformis[J].Journal of Zhejiang Sci-Tech University,2015,33-34(自科5):543.

备注/Memo

备注/Memo:
收稿日期: 2014-11-21
基金项目: 国家自然科学基金项目(31200110);浙江省高校生物学重中之重(一级)学科开放基金项目(2012D11);浙江理工大学科研启动基金项目(13042166-Y)
作者简介: 吴程雨(1990-),男,广西桂林人,硕士研究生,主要从事分子生物学和微生物发酵方面的研究
通信作者: 陈玮,E-mail:cw@zstu.edu.cn
更新日期/Last Update: 2015-09-14