|本期目录/Table of Contents|

[1].崔磊, 黎江, 刘辉, 吴红平, 李林芳, 郝方元, 金华君, 钱其军[J].浙江理工大学学报,2015,33-34(自科4):558-564.
 CUI Lei,LI Jiang,LIU Hui,et al.Effect of SelfExpression of SIRPαFc Fusion Protein on T Cell Function[J].Journal of Zhejiang Sci-Tech University,2015,33-34(自科4):558-564.
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崔磊, 黎江, 刘辉, 吴红平, 李林芳, 郝方元, 金华君, 钱其军()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第33-34卷
期数:
2015年自科4期
页码:
558-564
栏目:
出版日期:
2015-07-10

文章信息/Info

Title:
Effect of SelfExpression of SIRPαFc Fusion Protein on T Cell Function
文章编号:
1673-3851 (2015) 05-0712-06
作者:
1. 浙江理工大学生命科学院新元医学与生物技术研究所, 杭州 310018;2. 第二军医大学上海东方肝胆外科医院病毒基因治疗实验室, 上海 200438
Author(s):
CUI Lei LI Jiang LIU Hui WU Hongping LI Linfang HAO Fangyuan JIN Huajun QIAN Qijun
1. School of Life Science, Zhejiang Sci-Tech University, Hangzhou 310018, China;2. Eastern Hepatobiliary Surgical Hospital, the Second Military Medical University, Shanghai 200438, China
关键词:
CD47 T细胞 腺病毒 肝癌
分类号:
Q789
文献标志码:
A
摘要:
探讨携带SIRPαFc(SF)融合基因蛋白基因的重组腺病毒Ad35SF感染T细胞后对T细胞功能的影响。腺病毒Ad35 SF感染Jurkat T细胞后,通过Western blotting及ELISA方法检测细胞上清中SF融合蛋白的大小与表达量;通过流式分析技术检测细胞表面CD47的封闭情况;通过CCK8方法检测细胞的增殖与对肿瘤细胞的杀伤作用。结果表明,Ad35 SF构建成功,其感染Jurkat T细胞后,能在其内表达并分泌产生大小约为48 kD的SF蛋白,细胞上清中SF的表达量可到达19.1 μg/mL。Ad35 SF(MOI =1)感染Jurkat细胞18 h后,细胞CD47阳性比例从97.06%下降到15.32%;当Ad35 SF感染复数增加至5,10时,CD47阳性比率进一步降低至2.94%和1-73%。相对于空病毒对照组,Ad35 SF感染Jurkat细胞48 h后其增殖效果提高了29%,对肝癌细胞株Hep 3B的杀伤作用提升25.6%( p <0.01)。结果表明构建的重组腺病毒Ad35 SF能有效感染Jurkat细胞并在其内表达分泌SF蛋白,封闭细胞表面的CD47,同时显著提高Jurkat细胞的增殖能力和对肝癌细胞的杀伤作用。

参考文献/References:

[1] VernonWilson E F, Kee W J, Willis A C, et al. CD47  is a ligand for rat macrophage membrane signal regulatory  protein SIRP (OX41) and human SIRPalpha 1[J]. Eur J Immunol, 2000, 30(8): 21302137.[2] Barclay A N, Brown M H. The SIRP family of receptors and immune regulation[J]. Nat Rev Immunol, 2006, 6(6): 457-464.
[3] van den Berg T K, van der Schoot C E. Innate immune  ‘self’ recognition: a role for CD47SIRPalpha interactions in hematopoietic stem cell transplantation[J]. Trends Immunol, 2008, 29(5): 203-206.
[4] Majeti R, Chao M P, Alizadeh A A, et al. CD47 is an adverse prognostic factor and therapeutic antibody target on human acute myeloid leukemia stem cells[J]. Cell, 2009, 138(2): 286-299.
[5] Chao M P, Alizadeh A A, Tang C, et al. Therapeutic antibody  targeting of CD47 eliminates human acute lymphoblastic leukemia[J]. Cancer Res, 2011, 71(4): 1374-1384.
[6] Edris B, Weiskopf K, Volkmer A K, et al. Antibody therapy targeting the CD47 protein is effective in a model of aggressive metastatic leiomyosarcoma[J]. Proc Natl Acad Sci U S A, 2012, 109(17): 6656-6661.
[7] Brooke G, Holbrook J D, Brown M H, Barclay AN human lymphocytes interact directly with CD47 through a novel member of the signal regulatory protein (SIRP) family[J]. J Immunol, 2004, 173(4): 2562-2570.
[8] Irandoust M, Alvarez Zarate J, Hubeek I, et al. Engagement of SIRPalpha inhibits growth and induces programmed cell death in acute myeloid leukemia cells[J]. PLoS One, 2013, 8(1): e52143.
[9] Li Z, He L, Wilson K, et al. Thrombospondin1 inhibits TCRmediated T lymphocyte early activation[J]. J Immunol, 2001, 166(4): 2427-2436.
[10] Li  Z, Calzada M J, Sipes J M, et al. Interactions of thrombospondins with alpha4beta1 integrin and CD47 differentially modulate T cell behavior[J]. J Cell Biol, 2002, 157(3): 509-519.
[11] Kaur S, Kuznetsova S A, Pendrak M L, et al. Heparan sulfate modification of the transmembrane receptor CD47 is necessary for inhibition of T cell receptor signaling by thrombospondin1[J]. J Biol Chem, 2011, 286(17): 14991-15002.
[12] SotoPantoja D R, Terabe M, Ghosh A, et al. CD47 in the tumor microenvironment limits cooperation between antitumor Tcell immunity and radiotherapy[J]. Cancer Res, 2014, 74(23): 6771-6783.

相似文献/References:

[1]崔磊,黎江,刘辉,等.自表达SIRPα Fc融合蛋白对T细胞功能的影响[J].浙江理工大学学报,2015,33-34(自科5):712.
 CUI Lei,LI Jiang,LIU Hui,et al.Effect of SelfExpression of SIRPαFc Fusion Protein on T Cell Function[J].Journal of Zhejiang Sci-Tech University,2015,33-34(自科4):712.

备注/Memo

备注/Memo:
收稿日期: 2014-12-15
基金项目: 国家自然科学基金项目(81071850)
作者简介: 崔磊(1989-),男,山西运城人,硕士研究生,主要从事靶向基因—病毒治疗方面的研究
通信作者: 钱其军,E-mail:qianqj@sino-gene.com
更新日期/Last Update: 2015-09-24