|本期目录/Table of Contents|

[1]韩华锋,杨卫,陈菲菲,等.碳酸钙微球合成、修饰及作为基因载体的研究[J].浙江理工大学学报,2014,31-32(自科6):685-690.
 HAN Hua feng,YANG Wei,CHEN Fei fei,et al.Study on Synthesis and Modification of Calcium Carbonate Microparticles as Gene Carrier[J].Journal of Zhejiang Sci-Tech University,2014,31-32(自科6):685-690.
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碳酸钙微球合成、修饰及作为基因载体的研究()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第31-32卷
期数:
2014年自科6期
页码:
685-690
栏目:
(自科)生物与生命科学
出版日期:
2014-11-10

文章信息/Info

Title:
Study on Synthesis and Modification of Calcium Carbonate Microparticles as Gene Carrier
文章编号:
1673-3851 (2014) 06-0685-06
作者:
韩华锋 杨卫 陈菲菲 李泽豪 孔祥东
浙江理工大学, a. 生命科学学院; b. 生物材料与海洋生物资源研究所, 杭州 310018
Author(s):
HAN Huafeng YANG Wei CHEN Feifei LI Zehao KONG Xiangdong
a. School of Life Sciences; b. Institute of Biomaterials and Marine Biological Resources,Zhejiang Sci-Tech University, Hangzhou 310018, China
关键词:
碳酸钙微球 表面修饰 基因传递载体 基因治疗
分类号:
Q279
文献标志码:
A
摘要:
采用聚苯乙烯磺酸钠(PSS)作为调控剂,将其加入到氯化钙和碳酸钠溶液反应体系中成功制备出表面粗糙的碳酸钙微球(粒径平均约为510 nm),采用场发射扫描电镜(FE SEM)、红外光谱(FTIR)、X-射线衍射(XRD)和动态光散射(DLS)等对碳酸钙微球颗粒进行表征。MTT实验显示碳酸钙微球毒性具有浓度依赖性,在终浓度<1 mg/mL时,细胞存活率都可达60%以上,显示出很低的细胞毒性,具有良好的生物相容性。不同pH值条件下碳酸钙微球的降解实验结果显示碳酸钙微球在酸性pH下能够更快速地降解,说明碳酸钙微球能够响应酸性pH值,具有pH依赖性。将碳酸钙微球经PEI表面修饰后用于负载质粒DNA,通过琼脂糖凝胶电泳及体外转染实验结果证实碳酸钙微球能够有效负载质粒DNA,并可在癌细胞内成功表达发出绿色荧光。这些研究结果显示将碳酸钙微球作为一种安全的非病毒基因载体用于基因治疗领域具有潜在的应用价值。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期: 2013-02-14
基金项目: 国家自然科学基金(51272236,51172207,51002139);浙江省自然科学基金(Y207217);浙江理工大学521人才培养计划资助
作者简介: 韩华锋(1988-),女,安徽宿州人,硕士研究生,研究方向为生物材料方向
通信作者: 孔祥东,电子邮箱:kongxiangdong@gmail.com
更新日期/Last Update: 2014-11-19