|本期目录/Table of Contents|

[1]黄云仙,张风群,贾军祥,等.ILF3蛋白对雌激素受体beta的调控研究[J].浙江理工大学学报,2013,30(06):887-890.
　HUANG Yun xian,ZHANG Feng qun,JIA Jun xiang,et al.Research on Regulation of Estrogen Receptor Beta by ILF3 Protein[J].Journal of Zhejiang Sci-Tech University,2013,30(06):887-890.
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ILF3蛋白对雌激素受体beta的调控研究()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第30卷
期数:
2013年06期
页码:
887-890
栏目:
(自科)生物与生命科学
出版日期:
2013-11-10

文章信息/Info

Title:
Research on Regulation of Estrogen Receptor Beta by ILF3 Protein
文章编号:
1673-3851 (2013) 06-0887-04
作者:
黄云仙;  张风群;  贾军祥;  丁明;  赵辅昆
浙江理工大学生命科学学院, 杭州 310018
Author(s):
HUANG Yun xian;  ZHANG Feng qun;  JIA Jun xiang;  DING Ming;  ZHAO Fu kun
School of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China
关键词:
ILF3;  ERβ;  调控表达;  MTT
分类号:
Q291
文献标志码:
A
摘要:
雌激素在体内的生理作用通过雌激素受体(Estrogen Receptor)ERβ和ERα的介导来实现调控。ERβ在乳腺癌、前列腺癌、结肠癌和卵巢癌的发生、发展过程中具有调控作用。ILF3是RNA结合蛋白中的一员,参与细胞周期调控。凝胶阻滞实验(EMSA)证明了ILF3蛋白能结合ERβ mRNA 3 UTR。雌激素受体可介导雌激素实现其功能,ILF3蛋白可能通过与ERβ结合参与雌激素信号通路的调控。为探讨他们的关系以及调控机制,构建ILF3过表达的慢病毒载体,通过Western blot检测ERβ蛋白的表达情况。在蛋白水平上,ERβ蛋白的表达量随着ILF3蛋白量的升高而升高,ILF3对ERβ起正向调控。MTT实验检测ILF3基因表达对MCF 7细胞活力有影响。

参考文献/References:

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［8］ Reichman T W, Parrott A M, FierroMonti I, et al. Selective regulation of gene expression by nuclear factor 110, a member of the nf90 family of doublestranded rna binding proteins［J］. Journal of Molecular Biology, 2003, 332(1): 85-98.
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备注/Memo

备注/Memo:
收稿日期: 2012-12-27
作者简介: 黄云仙(1987-),女,浙江建德人,硕士研究生,主要从事肿瘤蛋白质组学研究。
通信作者: 丁明,E-mail:mingding@zstu.edu.cn
更新日期/Last Update: 2013-11-12