|本期目录/Table of Contents|

[1]韦敬航,杨力媛,郑洁,等.端粒酶RNA模板定点突变对肿瘤细胞 Bcap 37的作用研究[J].浙江理工大学学报,2013,30(05):753-757.
 WEI Jinghang,YANG Liyuan,ZHENG Jie,et al.Research on Effect of Telomerase RNA Template Sitespecific Mutagenesis on Cancer Cell Bcap37[J].Journal of Zhejiang Sci-Tech University,2013,30(05):753-757.
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端粒酶RNA模板定点突变对肿瘤细胞 Bcap 37的作用研究()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第30卷
期数:
2013年05期
页码:
753-757
栏目:
生物与生命科学
出版日期:
2013-09-10

文章信息/Info

Title:
Research on Effect of Telomerase RNA Template Sitespecific Mutagenesis on Cancer Cell Bcap37
文章编号:
1673-3851 (2013) 05-0753-05
作者:
韦敬航 杨力媛 郑洁 吕齐 何丹枫 刘小川
浙江理工大学生物工程研究所, 杭州 310018
Author(s):
WEI Jinghang YANG Liyuan ZHENG Jie Lv Qi HE Danfeng LIU Xiaochuan
Bioengineering Institute, Zhejiang Sci-Tech University, Hangzhou 310018, China
关键词:
人乳腺癌细胞株Bcap 37 端粒酶活性 端粒长度 细胞凋亡
分类号:
Q243
文献标志码:
A
摘要:
端粒酶在正常体细胞中极少表达,而在大多数人类恶性肿瘤和细胞系中高度表达,这暗示着端粒酶活性可能与肿瘤密切相关。本研究以人乳腺癌细胞株Bcap37接种裸鼠形成的肿瘤组织块为实验材料,通过检测端粒酶活性、端粒长度及细胞凋亡的情况,观察端粒酶RNA模板定点突变的作用。结果显示:MThTER组样本的端粒酶活性低于对照组,端粒长度短于对照组,细胞核形态不够稳定,有较多细胞凋亡的现象,而对照组细胞核形态稳定,较少发生细胞凋亡。以上结果提示着端粒酶RNA模板定点突变在一定程度上改变了人乳腺癌细胞株Bcap37的端粒酶活性、端粒长度及细胞凋亡发生率。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期: 2012-12-10
基金项目: 浙江省大学生科技创新活动计划(新苗人才计划)
作者简介: 韦敬航(1987-),男,广西宜州市人,硕士研究生,主要从事染色体端粒、端粒酶与细胞的癌变机制研究。
通信作者: 刘小川,电子邮箱:xcliu@zstu.edu.cn
更新日期/Last Update: 2013-10-23