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 HAN Wenzheng,WU Hu,LI Xiaoyan,et al.Study of Expression and Relevance of htra 2 in Macrophage and LPS[J].Journal of Zhejiang Sci-Tech University,2013,30(03):383-388.





Study of Expression and Relevance of htra 2 in Macrophage and LPS
1673-3851 (2013) 03-0383-06
韩文正 武虎 李晓艳 李红艳 姚韵靓 郭科妮 周秀梅
1. 浙江理工大学生命科学学院新元医学与生物技术研究所, 杭州 310018; 2. 浙江大学医学院免疫研究所, 杭州 310058
HAN Wenzheng WU Hu LI Xiaoyan LI Hongyan YAO Yunliang GUO Keni ZHOU Xiumei
1. Xinyuan Institute of Medicine and Biotechnology, School of Life Sciences,Zhejiang Sci-Tech University, Hangzhou 310018, China; 2. Institute of Immunology, School of Medicine, Zhejiang University, Hangzhou 310058, China
巨噬细胞 LPS VSV 免疫荧光 IL6
通过LPS(lipopolysaccharide)分别刺激树突状细胞和巨噬细胞,发现巨噬细胞中HtrA丝氨酸蛋白酶2(HtrA serine peptidase 2, htra2)基因的表达随着刺激时间的延长呈现下调的变化趋势,提示巨噬细胞中的htra2受到LPS的调控。为了进一步证实LPS刺激的特异性,实验建立VSV(vesicular stomatitis virus)病毒感染巨噬细胞模型,采用荧光定量PCR,免疫荧光方法分别从 htra2的蛋白水平和定位上研究 htra2 的变化与LPS刺激巨噬细胞产生效应的相关性;构建了 htra2 基因过表达的质粒pKH3 htra2 HA,采用ELISA,Western Blot及荧光定量PCR等方法,检测过表达 htra2 对巨噬细胞产生和分泌炎症因子IL 6(Interleukin 6)及IL 1b(Interleukin 1b)的影响。实验结果表明: htra2 仅在LPS刺激巨噬细胞的体系中受到调控,且过表达的 htra2 会上调巨噬细胞IL 1b和IL 6的表达水平。


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收稿日期: 2012-10-08
基金项目: 国家自然科学基金(81272687);科研启动基金(1204807 Y)
作者简介: 韩文正(1986-),男,安徽萧县人,硕士研究生,主要从事肿瘤的靶向基因治疗研究。
通信作者: 周秀梅,电子邮箱:zhouxiumei824@163.com
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