|本期目录/Table of Contents|

[1]李敏,曹慧,钱文斌. Hsp90抑制剂BIIB021对人急性T细胞白血病Molt4细胞周期和凋亡的影响[J].浙江理工大学学报,2012,29(04):589-593.
 LI Min,CAO Hui,QIAN Web bin.Effects of Hsp90 Inhibitor BIIB021 on Apoptosis and Cell Cycle inHuman TCell Acute Lymphocytic Leukemia Cell Line Molt4[J].Journal of Zhejiang Sci-Tech University,2012,29(04):589-593.
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 Hsp90抑制剂BIIB021对人急性T细胞白血病Molt4细胞周期和凋亡的影响()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第29卷
期数:
2012年04期
页码:
589-593
栏目:
生物与生命科学
出版日期:
2012-08-28

文章信息/Info

Title:
Effects of Hsp90 Inhibitor BIIB021 on Apoptosis and Cell Cycle inHuman TCell Acute Lymphocytic Leukemia Cell Line Molt4
文章编号:
16733851 (2012) 04058905
作者:
 李敏1 曹慧1 钱文斌12
 1. 浙江理工大学生命科学学院 新元医学与生物技术研究所, 杭州 310018;2. 浙江大学医学院附属第一医院 血液病研究所, 杭州 310003
Author(s):
LI Min1 CAO Hui1 QIAN Webbin12
 1. Xinyuan Institute of Medicine and Biotechnology, School of Life Sciences, Zhejiang SciTechUniversity, Hangzhou 310018, China; 2. Institute of Hematology, the First Affiliated Hospital,School of Medicine, Zhejiang University, Hangzhou 310003, China
关键词:
 Hsp90抑制剂 BIIB021 急性T淋巴细胞白血病 细胞凋亡 细胞周期
分类号:
Q733.7
文献标志码:
A
摘要:
    为了研究Hsp90抑制剂BIIB021对人急性T淋巴细胞白血病细胞株Molt4增殖和凋亡的影响,采用MTT法检测了BIIB021对Molt4细胞增殖的影响,Hoechst33258荧光染色法检测细胞凋亡形态学变化,流式细胞术检测药物作用前后细胞周期和凋亡的改变,Western blotting 检测BIIB021对细胞周期和凋亡相关蛋白的影响。结果显示:BIIB021以时间和剂量依赖性方式抑制Molt4细胞增殖,48、72 h的IC50分别为384.6 nmol/L和301.8 nmol/L;Hoechst33258染色可观察到细胞内出现凋亡小体,且随着药物浓度增大,凋亡小体的数目也逐渐增多;流式结果同样显示,随着药物浓度升高,细胞凋亡率逐渐上升,400 nmol/L BIIB021可诱导细胞凋亡率达34.4%;另外,BIIB021可显著阻滞细胞于G0/G1期;Western blotting显示BIIB021激活Caspase8、9、3和PARP,下调CDK4/6,上调p21和p18,并能明显抑制Akt和p65。由此表明,BIIB021能激活死亡受体途径和线粒体途径诱导Molt4细胞凋亡,并通过影响CDK4/6和p21、p18使得Molt4细胞被阻滞于G0/G1期,抑制细胞增殖;BIIB021对Molt4的Akt和NFκB也有抑制作用。

参考文献/References:

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备注/Memo

备注/Memo:
 收稿日期: 2011-10-25
基金项目: 浙江省自然科学基金杰出青年团队项目(R2090392);浙江理工大学科研启动基金项目(1016834-Y)
作者简介: 李敏(1987-),女,河南中牟人,硕士研究生,主要从事肿瘤的分子靶向治疗研究。
通讯作者: 钱文斌,电子邮箱:qianwenb@yahoo.com.cn
更新日期/Last Update: