|本期目录/Table of Contents|

[1]梁天祥,谌贺宽子,陈磊,等. 融合蛋白sCARTSP1原核表达纯化及诱导白血病细胞K562凋亡的研究[J].浙江理工大学学报,2012,29(04):584-588.
 LIANG Tian xiang,SHEN HE Kuan zi,CHEN Lei,et al. Prokaryotic Expression and Purification of sCARTSP1, andInduced Apoptosis in Leukemic Cells k562[J].Journal of Zhejiang Sci-Tech University,2012,29(04):584-588.
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 融合蛋白sCARTSP1原核表达纯化及诱导白血病细胞K562凋亡的研究()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第29卷
期数:
2012年04期
页码:
584-588
栏目:
生物与生命科学
出版日期:
2012-08-28

文章信息/Info

Title:
 Prokaryotic Expression and Purification of sCARTSP1, andInduced Apoptosis in Leukemic Cells k562
文章编号:
16733851 (2012) 04058405
作者:
 梁天祥 谌贺宽子 陈磊 武虎 唐斌
 浙江理工大学生命科学学院新元医学与生物技术研究所, 杭州 310018
Author(s):
 LIANG Tianxiang SHENHE Kuanzi CHEN Lei WU Hu TANG Bin
 Xinyuan Institute of Medicine and Biotechnology, School of Life Sciences,Zhejiang SciTech University, Hangzhou 310018, China
关键词:
 TSP1 sCARTSP1融合蛋白 原核表达 白血病细胞
分类号:
Q789
文献标志码:
A
摘要:
     根据thrombospondin1(TSP1)氨基酸序列(RFYVVMWK),以已有的腺病毒受体sCAR为模板,将8个氨基酸对应基因核苷酸通过PCR扩增于sCAR之后,得到sCARTSP1,连接到表达载体pQE30上,转化大肠杆菌M15后获得工程茵。该菌株经IPTG诱导后,高效表达出带有组氨酸标签以包涵体形式存在的融合蛋白sCARTSP1。包涵体经过尿素变性溶解、PBS稀释复性、Ni离子亲和层析柱纯化,获得目的蛋白。SDSPAGE分析表明,有一条明显的特异性蛋白条带。同时细胞实验结果表明融合蛋白sCARTSP1对白血病细胞K562有明显凋亡作用。

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备注/Memo

备注/Memo:
 收稿日期: 2011-11-03
基金项目: 国家自然科学基金项目(30801379);浙江理工大学科研启动基金项目(1016834-Y)
作者简介: 梁天祥(1986-),男,山西大同人,硕士研究生,主要从事肿瘤的靶向基因病毒治疗研究。
通讯作者: 唐斌,电子邮箱:tangbin1111@yahoo.com.cn
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