|本期目录/Table of Contents|

[1]张海花b,王婕a,高慧,等. 二步超离心法分离纯化人血浆Apo B100及兔抗人Apo B100抗血清制备[J].浙江理工大学学报,2012,29(01):115-118.
 ZHANG Hai\|huab,WANG Jiea,GAO Hui,et al. Preparation of Apo B100 Rabbit Anti\|Human Antiserum[J].Journal of Zhejiang Sci-Tech University,2012,29(01):115-118.
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 二步超离心法分离纯化人血浆Apo B100及兔抗人Apo B100抗血清制备()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第29卷
期数:
2012年01期
页码:
115-118
栏目:
生物与生命科学
出版日期:
2012-02-28

文章信息/Info

Title:
 Preparation of Apo B100 Rabbit Anti\|Human Antiserum
文章编号:
16733851 (2012) 01011504
作者:
 张海花1b 王婕1a 高慧2 付彩云1a 童富淡1b
 1. 浙江理工大学,a. 生命科学学院,b. 浙江省家蚕生物反应器和生物医药重点实验室,杭州 310018;2. 浙江省畜牧技术服务中心,杭州 310021
Author(s):
 ZHANG Hai\|hua1b WANG Jie1a GAO Hui2 FU Cai\|yun1a TONG Fu\|dan1b
 1. Zhejiang Sci\|Tech University, a. School of Life Sciences, b. Zhejiang Provincial Key Laboratory ofSilkworm Bioreactor and Biomedicine, Hangzhou 310018, China; 2. Zhejiang Provincial AnimalHusbandry Technology Service Center, Hangzhou 310021, China
关键词:
 Apo B100 超速离心 ELISA 双向免疫扩散
分类号:
Q33
文献标志码:
A
摘要:
    建立一种从人血浆中分离纯化Apo B100的方法,并制备兔抗人Apo B100抗血清。将血浆用溴化钾调成1.019 g/mL的密度液进行差分超速离心,将分离产物在溴化钾不连续密度梯度液(1.006~1.210 g/mL)中进行等密度超速离心。用G\|100葡聚糖凝胶柱纯化Apo B100,以ELISA双抗体夹心法检测浓度,真空冷冻干燥。取冻干粉与弗氏佐剂混合后免疫新西兰白兔,制备兔抗人Apo B100抗血清,利用双向免疫扩散检测抗原纯度与抗血清效价。 结果是一步超离法分离的Apo B100最高浓度为0.91 μg/mL;二步超离法分离的Apo B100最高浓度为1.40 μg/mL,对应的抗血清效价为1∶64。结果表明二步超离心法提高了人血浆Apo B100的分离效果。

参考文献/References:

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备注/Memo

备注/Memo:
 收稿日期: 2011-06-29
基金项目: 国家自然科学基金项目(31000496);浙江省教育厅科研项目(Y200909740)
作者简介: 张海花(1981-) ,女,山东巨野人,硕士,讲师,主要从事生化与分子生物学研究。
通讯作者: 童富淡,电子邮箱:fdtong@zstu.edu.cn
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