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[1]马会彦a,陈钊,李杰,等. 人芳香基硫酸酯酶(HSulf1)基因的原核表达及酶活初步研究[J].浙江理工大学学报,2011,28(05):789-794.
 MA Hui yana,CHEN Zhao,LI Jie,et al.Prokaryotic Expression and Enzyme Activity Measurement of the Human Arylsulfatase(HSulf1)[J].Journal of Zhejiang Sci-Tech University,2011,28(05):789-794.
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 人芳香基硫酸酯酶(HSulf1)基因的原核表达及酶活初步研究()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第28卷
期数:
2011年05期
页码:
789-794
栏目:
生物与生命科学
出版日期:
2011-10-30

文章信息/Info

Title:
Prokaryotic Expression and Enzyme Activity Measurement of the Human Arylsulfatase(HSulf1)
文章编号:
16733851 (2011) 05078906
作者:
 马会彦1a 陈钊2 李杰2 莫敏俐2 谢国良1b 盛清1a
 1. 浙江理工大学生命科学学院, a. 生物化学研究所, b. 新元医学与生物技术研究所, 杭州 310018; 2. 清华大学生命科学学院分子酶学实验室, 北京 100084
Author(s):
MA Huiyan1a CHEN Zhao2 LI Jie2 MO Minli2 XIE Guoliang1b SHENG Qing1a
 1. School of Life Sciences, Zhejiang SciTech University, a. Biochemistry Institute,b. Xinyuan Institute of Medicine and Biotechnology, Hangzhou 310018, China; 2. MolecularEnzymology Laboratory, School of Life Sciences, Tsinghua University, Beijing 100084, China
关键词:
 芳香基硫酸酯酶 HSulf1 原核表达 酶活性
分类号:
Q789
文献标志码:
A
摘要:
    人芳香基硫酸酯酶(HSulf1)是一类分泌型蛋白,能在中性条件下改变硫酸肝素蛋白聚糖(HSPGs)的硫酸化状态,从而影响多种信号分子与其相应受体的结合,进而影响细胞信号通路。鉴于HSulf1具有重要的潜在应用价值,为获得大量纯化蛋白以探索其功能与应用,文章将HSulf1功能域基因片段与pGEX6p1表达载体连接构建成重组质粒,在原核表达系统BL21中表达、分离纯化了HSulf1的功能域片段,并以4Mus为底物用荧光光度法进行了酶活性的测定。结果表明,原核表达能得到电泳纯级的HSulf1纯化蛋白,但其具有酶活性的条件需要进一步探索。

参考文献/References:

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备注/Memo

备注/Memo:
 收稿日期: 2010-11-02
基金项目: 国家自然科学基金项目(30770475);国家863重点项目(2007AA021703);浙江省自然基金面上项目(Y3090228)
作者简介: 马会彦(1982-),男,山东济宁人,硕士研究生,主要从事生物化学与分子生物学研究。
通讯作者: 盛清,电子邮箱:csheng@zstu.edu.cn
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