|本期目录/Table of Contents|

[1]张璇,刘佳,马蕾娜,等. 稳定下调SIRT1基因表达的肝癌细胞系的构建[J].浙江理工大学学报,2011,28(05):778-782.
　ZHANG Xuan,LIU Jia,MA Lei na,et al. Establishment of Stable SIRT1 GeneDownregulated Hepatocarcinoma Cell Lines by Lentiviral Vector[J].Journal of Zhejiang Sci-Tech University,2011,28(05):778-782.
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 稳定下调SIRT1基因表达的肝癌细胞系的构建()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第28卷
期数:
2011年05期
页码:
778-782
栏目:
(自科)生物与生命科学
出版日期:
2011-10-30

文章信息/Info

Title:
 Establishment of Stable SIRT1 GeneDownregulated Hepatocarcinoma Cell Lines by Lentiviral Vector
文章编号:
16733851 (2011) 05077805
作者:
 张璇1;  刘佳2;  马蕾娜2;  胡徐庞1;  刘立1;  钱程1
 1. 浙江理工大学生命科学院, 杭州 310018； 2. 重庆市西南医院， 重庆 400038
Author(s):
 ZHANG Xuan1;  LIU Jia2;  MA Leina 2;  HU Xupang1;  LIU Li1;  QIAN Cheng1
 1. School of Life Sciences, Zhejiang SciTech University, Hangzhou 310018, China; 2. Southwest Hospital, Chongqing 400038, China
关键词:
 ; SIRT1;  嘌呤霉素;  慢病毒载体;  肝癌细胞系
分类号:
Q279
文献标志码:
A
摘要:
    SIRT1(silent information regulator 1）与多种肿瘤的发病过程关系密切，但是其在肝癌中的作用尚不明确。为了研究SIRT1与肝癌的关系，通过构建慢病毒载体，病毒转染和包装，嘌呤霉素的筛选等实验方法，成功构建能稳定下调SIRT1基因表达的肝癌细胞系Hep3B和PLC/PRF/5，为后期进一步研究SIRT1在肝癌发病过程中的功能奠定了基础。

参考文献/References:

 ［1］ Bourguignon, Xia Weiliang, Gabriel Wong, et al. Hyaluronanmediated CD44 interaction with p300 and SIRT1 regulates betacatenin signaling and NFkappaBspecific transcription activity leading to MDR1 and BclxL gene expression and chemoresistance in breast tumor cells［J］. J Biol Chem, 2009, 284(5): 26572671.
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［5］ Kim E J, Kho J H, Kang M R, et al. Active regulator of SIRT1 cooperates with SIRT1 and facilitates suppression of p53 activity［J］. Mol Cell, 2007, 28: 277290.
［6］ Zhao W, Kruse J P, Tang Y, et al. Negative regulation of the deacetylase SIRT1 by DBC1［J］. Nature, 2008, 451: 587590.
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［8］ Huffman D M, Grizzle W E, Bamman M M, et al. SIRT1 is significantly elevated in mou se and human prostate cancer［J］. Cancer Res, 2007, 67: 66126618.
［9］ Liu G, Yuan X, Zeng Z, et al. Analysis of gene expression and chemoresistance of CD133+ cancer stem cells in glioblastoma［J］. Mol Cancer, 2006, 6: 67
［10］ Yang D, Buchholz F, Huang Z, et al. Short RNA duplexes produced by hydrolysis with Escherichia coli RNase Ⅲ mediate effective RNA interference in mammalian cells［J］. Proc Natl Acad Sci USA, 2002, 99(15): 99429947.
［11］ Gondai T, Yamaguchi K, MiyanoKurosaki N, et al. Shorthairpin RNAs synthesized by T7 phage polymerase do not induce interferon［J］. Nucleic Acids Res, 2008, 36(16): e18.

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备注/Memo

备注/Memo:
 收稿日期： 2010-12-20
基金项目： 国家重点基础发展计划资助项目（973）（2010CB529406）；重庆市自然科学基金计划资助项目(CSTC 09BB5024)
作者简介： 张璇(1986-)，男，江西新余人，硕士研究生，主要从事肿瘤基因治疗方面的研究。
通讯作者： 刘立,电子邮箱:liuli7001@yahoo.com.cn
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