|本期目录/Table of Contents|

[1]张璇,刘佳,马蕾娜,等. 稳定下调SIRT1基因表达的肝癌细胞系的构建[J].浙江理工大学学报,2011,28(05):778-782.
 ZHANG Xuan,LIU Jia,MA Lei na,et al. Establishment of Stable SIRT1 GeneDownregulated Hepatocarcinoma Cell Lines by Lentiviral Vector[J].Journal of Zhejiang Sci-Tech University,2011,28(05):778-782.
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 稳定下调SIRT1基因表达的肝癌细胞系的构建()
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浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第28卷
期数:
2011年05期
页码:
778-782
栏目:
生物与生命科学
出版日期:
2011-10-30

文章信息/Info

Title:
 Establishment of Stable SIRT1 GeneDownregulated Hepatocarcinoma Cell Lines by Lentiviral Vector
文章编号:
16733851 (2011) 05077805
作者:
 张璇1 刘佳2 马蕾娜2 胡徐庞1 刘立1 钱程1
 1. 浙江理工大学生命科学院, 杭州 310018; 2. 重庆市西南医院, 重庆 400038
Author(s):
 ZHANG Xuan1 LIU Jia2 MA Leina 2 HU Xupang1 LIU Li1 QIAN Cheng1
 1. School of Life Sciences, Zhejiang SciTech University, Hangzhou 310018, China; 2. Southwest Hospital, Chongqing 400038, China
关键词:
 SIRT1 嘌呤霉素 慢病毒载体 肝癌细胞系
分类号:
Q279
文献标志码:
A
摘要:
    SIRT1(silent information regulator 1)与多种肿瘤的发病过程关系密切,但是其在肝癌中的作用尚不明确。为了研究SIRT1与肝癌的关系,通过构建慢病毒载体,病毒转染和包装,嘌呤霉素的筛选等实验方法,成功构建能稳定下调SIRT1基因表达的肝癌细胞系Hep3B和PLC/PRF/5,为后期进一步研究SIRT1在肝癌发病过程中的功能奠定了基础。

参考文献/References:

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备注/Memo

备注/Memo:
 收稿日期: 2010-12-20
基金项目: 国家重点基础发展计划资助项目(973)(2010CB529406);重庆市自然科学基金计划资助项目(CSTC 09BB5024)
作者简介: 张璇(1986-),男,江西新余人,硕士研究生,主要从事肿瘤基因治疗方面的研究。
通讯作者: 刘立,电子邮箱:liuli7001@yahoo.com.cn
更新日期/Last Update: