|本期目录/Table of Contents|

[1]梁晨,单娟娟,沈俊杰,等. 肝癌细胞系Huh7中肿瘤干细胞分离及鉴定[J].浙江理工大学学报,2011,28(04):601-605.
 LIANG Chen,SHAN Juan juan,SHENG Jun jie,et al. Isolation and Characterization of Cancer Stem Cells from a Human Hepatocellular Carcinoma Cell Lines Huh7[J].Journal of Zhejiang Sci-Tech University,2011,28(04):601-605.
点击复制

 肝癌细胞系Huh7中肿瘤干细胞分离及鉴定()
分享到:

浙江理工大学学报[ISSN:1673-3851/CN:33-1338/TS]

卷:
第28卷
期数:
2011年04期
页码:
601-605
栏目:
(自科)生物与生命科学
出版日期:
2011-08-31

文章信息/Info

Title:
 Isolation and Characterization of Cancer Stem Cells from a Human Hepatocellular Carcinoma Cell Lines Huh7
文章编号:
16733851 (2011) 04060204
作者:
 梁晨 单娟娟 沈俊杰 刘立 钱程
 浙江理工大学生命科学学院, 杭州 310018
Author(s):
 LIANG Chen SHAN Juanjuan SHENG Junjie LIU Li QIAN Cheng
 Shool of Life Sciences, Zhejiang SciTech University, Hangzhou 310018, China
关键词:
 肿瘤干细胞 肝癌(干)细胞球 生长因子 EpCAM
分类号:
Q786
文献标志码:
A
摘要:
    利用肿瘤干细胞具有自我更新能力的特点,首次采用悬浮培养技术从Huh7细胞系中得到肝癌(干)细胞球,并通过RT\|PCR、流式细胞术和免疫荧光等方法检测它们的干细胞标记和分化标记。结果显示Huh7(干)细胞球的干性相关基因表达上调;分化相关基因表达下调;流式细胞仪检测结果表明88.82%的Huh7(干)细胞球表达EpCAM基因,与正常Huh7细胞相比表达上调。表明该方法具有富集肝癌干细胞的效果,为以后肝癌干细胞建立更高效的分离方法提供了一种新的技术方法。

参考文献/References:

 [1] Clarke M F, Dick J E, Dirks P B, et al. Cancer stem cells\|perspectives on current status and future directions: AACR workshop on cancer stem cells[J]. Cancer Res, 2006, 66(19): 9339\|9344.
[2] Reya T, Morrison S J, Clarke M F, et al. Stem cells, cancer, and cancer stem cells[J]. Nature, 2001, 414: 105\|111.
[3] Bonnet D, Dick J E. Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell[J]. Nat Med, 1997, 3(7): 730\|737.
[4] Al\|Hajj M, Wicha M S, Benito\|Hernandez A, et al. Prospective identification of tumorigenic breast cancer cells[J]. Proc Natl Acad Sci U S A, 2003, 100(7): 3983\|3988.
[5] Kim C F, Jackson E L, Woolfenden A E, et al. Identification of bronchioalveolar stem cells in normal lung and lung cancer[J]. Cell, 2005, 121(6):823\|835.
[6] Yu S C, Ping Y F, Yi L, et al. Isolation and characterization of cancer stem cells from a human glioblastoma cell line U87[J]. Cancer Lett, 2008, 265(1): 124\|134.
[7] Zhou S, Schuetz J D, Bunting K D, et al. The ABC transporter Bcrp1/ABCG2 is expressed in a wide variety of stem cells and is a molecular determinant of the side\|population phenotype[J]. Nat Med, 2001, 7(9): 1028\|1034.
[8] Yang Z F, Ho D W, Ng M N, et al. Significance of CD90+ cancer stem cells in human liver cancer[J]. Cancer Cell, 2008, 13(2): 153\|166.
[9] Yamashita T, Ji J, Budhu A, et al. EpCAM\|positive hepatocellular carcinoma cells are tumor\|initiating cells with stem/progenitor cell features[J]. Gastroenterology, 2009, 136(3): 1012\|1024.
[10] Haraguchi N, Utsunomiya T, Inoue H, et al. Characterization of a side population of cancer cells from human gastrointestinal system[J]. Stem Cells, 2006, 24(3): 506\|513.
[11] Reynolds B A, Weiss S. Clonal and population analyses demonstrate that an EGF\|responsive mammalian embryonic CNS precursor is a stem cell[J]. Dev Biol, 1996, 175(1): 1\|13.
[12] Dontu G, Abdallah W M, Foley J M, et al. In vitro propagation and transcriptional profiling of human mammary stem/progenitor cells[J]. Genes Dev, 2003, 17(10): 1253\|1270.
[13] Toma J G, Akhavan M, Fernandes K J, et al. Isolation of multipotent adult stem cells from the dermis of mammalian skin[J]. Nat Cell Biol, 2001, 3(9): 778\|784.
[14] Tsuchiya A, Heike T, Fujino H, et al. Long\|term extensive expansion of mouse hepatic stem/progenitor cells in a novel serum\|free culture system[J]. Gastroenterology, 2005, 128(7): 2089\|2104.
[15] Kimura O, Takahashi T, Ishii N, et al. Characterization of the epithelial cell adhesion molecule (EpCAM)+ cell population in hepatocellular carcinoma cell lines[J]. Cancer Sci, 2010, 101(10): 2145\|2155.

相似文献/References:

[1]陈智杰a,赵晓丽a,徐畅b,等. 有机颜料微胶囊在涂料染色中的应用及其染色效果分析[J].浙江理工大学学报,2013,30(01):1.
 CHEN Zhi jiea,ZHAO Xiao lia,XU Changb,et al. Application of Organic Pigment Microcapsule in Pigment Dyeingand Its Dyeing Results[J].Journal of Zhejiang Sci-Tech University,2013,30(04):1.
[2]詹永娟,谢维斌,姜晓云,等. 织物液态水传递性能的自动检测技术及应用[J].浙江理工大学学报,2013,30(01):6.
 ZHAN Yong juan,XIE Wei bin,JIANG Xiao yun,et al. Technology and Application of the Automatic Detection inTesting Liquid Transport Properties of Textiles[J].Journal of Zhejiang Sci-Tech University,2013,30(04):6.
[3]孙麒. 基于极大似然估计的织物图像分割[J].浙江理工大学学报,2013,30(01):12.
 SUN Qi. Textile Image Segmentation Based on theMaximum Likelihood Algorithm[J].Journal of Zhejiang Sci-Tech University,2013,30(04):12.
[4]巫静a,田彦杰b,汪澜a,等. 基于SVM理论的涤纶织物分散染料上染率模型研究[J].浙江理工大学学报,2013,30(01):21.
 WU Jinga,TIAN Yan jieb,WANG Lana,et al. Research on Modeling of DyeUptake Rate for Disperse Dyes onPolyester Fibers Based on SVM[J].Journal of Zhejiang Sci-Tech University,2013,30(04):21.
[5]周昊,徐英莲,齐素梅.微孔结构改性涤纶/棉混纺针织物服用性能的研究[J].浙江理工大学学报,2013,30(01):21.
 ZHAO Hao,XU Ying lian,QI Su mei. Research on Using Knitted Blend Fabric of Modified Polyesterby Cellular Structure and Cotton for Wearing[J].Journal of Zhejiang Sci-Tech University,2013,30(04):21.
[6]孙佳英,李艳清,章斐燕,等. 纺织结构复合材料铺层顺序设计与力学性能分析[J].浙江理工大学学报,2013,30(01):27.
 SUN Jia ying,LI Yan qing,ZHANG Fei yan,et al. Study on Layer Sequence Design and Mechanical Propertiesof Textile Structure Composites[J].Journal of Zhejiang Sci-Tech University,2013,30(04):27.
[7]丁源维,王騊,姚菊明,等. 静电纺制备TiO2/PVA复合纳米纤维及其光催化性能研究[J].浙江理工大学学报,2013,30(01):31.
 DING Yuan wei,WANG Tao,YAO Ju ming,et al. Photocatalytic Performance Investigation of TiO2/PVANanofibers Prepared by Electrospinning[J].Journal of Zhejiang Sci-Tech University,2013,30(04):31.
[8]章梦洁,伍仲,方园. 涤棉混纺织物阻燃性能的实验分析[J].浙江理工大学学报,2013,30(01):36.
 ZHANG Meng jie,WU Zhong,FANG Yang. Experimental Study on the PolyesterCotton BlendedFabrics Flame Retardancy[J].Journal of Zhejiang Sci-Tech University,2013,30(04):36.
[9]胡觉亮a,孔云鹏b,韩曙光a,等. 基于随机需求的服装供应链回购契约研究[J].浙江理工大学学报,2013,30(01):40.
 HU Jue lianga,KONG Yun pengb,HAN Shu guanga,et al. Study on Repurchase Contract in a Fashion Chainwith the Stochastic Demand[J].Journal of Zhejiang Sci-Tech University,2013,30(04):40.
[10]毛雯,阎玉秀. 针织服装供应商评价指标体系构建与权重确定[J].浙江理工大学学报,2013,30(01):46.
 MAO Wen,YAN Yu xiu. Building Evaluation Index System for Knitting Clothing Suppliersand Determining the Weight[J].Journal of Zhejiang Sci-Tech University,2013,30(04):46.
[11]路巧然,吴欣欣,贾晓渊,等. 卵巢癌病人腹水细胞的培养及其分化研究[J].浙江理工大学学报,2011,28(05):809.
 LU Qiao ran,WU Xin xin,JIA Xia yuan,et al. Research on the Culture and Differentiation of Ovarian Cancer Ascites Cells[J].Journal of Zhejiang Sci-Tech University,2011,28(04):809.

备注/Memo

备注/Memo:
 收稿日期: 2010-11-12
基金项目: 国家自然科学基金面上项目(30872984);重庆市自然科学基金计划项目(CSTC 09BB5024)
作者简介: 梁晨(1986-),女,山东济南人,硕士研究生,主要从事肿瘤基因病毒治疗方面的研究。
通讯作者: 刘立,E\|mail:liuli7001@yahoo.com.cn
更新日期/Last Update: